Instituto de Diagnóstico y Referencia Epidemiológicos, Secretaria de Salud, México D.F., Mexico 11340.
Parasitol Res. 2012 Oct;111(4):1599-605. doi: 10.1007/s00436-012-2998-3. Epub 2012 Jun 30.
A comparison was made of the effects of levamisole, the bacterial fractions of Staphylococcus, and Freund's adjuvant on the immunization of rats with the excretory and secretory antigens of Trichinella spiralis muscle larvae. Wistar rats were immunized with the antigen and a saline solution, levamisole (LV), Staphylococcus (ST), or Freund's adjuvant (FA). After immunization, rats were infected, and the parasite burden at muscular phase was calculated for each group. Levels of IgG1 and IgG2 antibodies, as well as levels of two cytokines, IL-4 and IFN-γ, were evaluated during the immunization and postinfection periods. Differences were found in the kinetics of antibody production between groups (p < 0.01). In all cases, there was reactivity with the main 45-, 50-, and 55-kDa antigens of Trichinella muscle larvae. Immunization with FA and ST enhanced the production of IgG1, but only FA showed a significant increase in the production of IFN-γ (p < 0.01), resulting in 86% protection against the infection. In contrast, only 60-70% protection was attained in the ST and LV groups (p < 0.01). These data support the idea that levamisole and Staphylococcus can be used as adjuvant to enhance the humoral response and, at the same time, demonstrate that IFN-γ could be involved in protection against Trichinella.
比较了左旋咪唑、金黄色葡萄球菌细菌成分和弗氏佐剂对旋毛虫肌肉幼虫排泄分泌抗原免疫大鼠的作用。用抗原和生理盐水、左旋咪唑（LV）、金黄色葡萄球菌（ST）或弗氏佐剂（FA）免疫 Wistar 大鼠。免疫后感染，计算每组的寄生虫负荷。在免疫和感染后期间评估 IgG1 和 IgG2 抗体以及两种细胞因子 IL-4 和 IFN-γ 的水平。发现抗体产生的动力学在各组之间存在差异（p <0.01）。在所有情况下，都与旋毛虫肌肉幼虫的主要 45、50 和 55 kDa 抗原发生反应。FA 和 ST 的免疫增强了 IgG1 的产生，但只有 FA 显示 IFN-γ 的产生显著增加（p <0.01），对感染的保护率达到 86%。相比之下，ST 和 LV 组的保护率仅为 60-70%（p <0.01）。这些数据支持了左旋咪唑和金黄色葡萄球菌可用作佐剂来增强体液反应的观点，同时表明 IFN-γ可能参与对旋毛虫的保护。
