Kaspar Ursula, von Lützau Knut, Schlattmann Andreas, Rösler Uwe, Köck Robin, Becker Karsten
Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.
Institute for Animal Hygiene and Environmental Health, FU Berlin, Department of Veterinary Medicine, Berlin, Germany.
One Health. 2019 Apr 1;7:100091. doi: 10.1016/j.onehlt.2019.100091. eCollection 2019 Jun.
Colonization with multidrug-resistant organisms (MDROs) belonging to the genus and the order poses a particular threat to populations at risk. While previous studies focused on MDRO carriage among livestock or companion animals, respective epidemiological data on the general equine population are limited. Here, carriage of methicillin-resistant (MRSA) and extended spectrum beta-lactamase (ESBL) producing (ESBL-E) in non-hospitalized horses living on private farms in the rural area in Northwest Germany was assessed. Intranasal and perianal swab samples were cultured on solid chromogenic media directly and after enrichment in tryptic soy broth, respectively. isolates were -typed, MRSA and ESBL-E were further classified by phenotypic and molecular methods. Additionally, a subgroup of the first 20 samples was used to isolate and characterize staphylococci other than . Among 223 horses, fifteen (6.8%) carried . Two isolates were identified as MRSA (0.9% of all horses, -positive) and classified as types t011 and t6867, both known as members of the livestock-associated MRSA MLST clonal complex 398. Nine horses (4.0%) were colonized by ESBL- positive for and/or . ESBL-E carriage was associated with prior antibiotic treatment (4/31 vs. 5/183; = 0.0362) and veterinary examinations (4/31 vs. 5/183; p = 0.0362). In the subgroup, nine different staphylococcal species other than were found. The high prevalence of ESBL- in non-hospitalized horses underlines the necessity to raise awareness for strain dissemination across different hosts in order to do justice to the "One Health" concept.
属于该属和该目的多重耐药菌（MDROs）的定植对高危人群构成了特殊威胁。虽然先前的研究集中在牲畜或伴侣动物中的MDRO携带情况，但关于一般马群的相应流行病学数据有限。在此，对生活在德国西北部农村地区私人农场的非住院马匹中耐甲氧西林金黄色葡萄球菌（MRSA）和产超广谱β-内酰胺酶（ESBL）肠杆菌科细菌（ESBL-E）的携带情况进行了评估。鼻内和肛周拭子样本分别直接在固体显色培养基上培养以及在胰蛋白胨大豆肉汤中富集后培养。对分离株进行血清型分型，通过表型和分子方法对MRSA和ESBL-E进行进一步分类。此外，使用前20个样本的一个亚组来分离和鉴定除金黄色葡萄球菌外的葡萄球菌。在223匹马中，15匹（6.8%）携带金黄色葡萄球菌。两株分离株被鉴定为MRSA（占所有马匹的0.9%，金黄色葡萄球菌阳性），并分类为t011和t6867型，这两种类型均为与牲畜相关的MRSA多位点序列分型克隆复合体398的成员。9匹马（4.0%）被ESBL-E定植，对blaCTX-M和/或blaTEM呈阳性。ESBL-E携带与先前的抗生素治疗（4/31 vs. 5/183；P = 0.0362）和兽医检查（4/31 vs. 5/183；p = 0.0362）相关。在该亚组中，发现了9种除金黄色葡萄球菌外的不同葡萄球菌物种。非住院马匹中ESBL-E的高流行率强调了提高对菌株在不同宿主间传播的认识的必要性，以便践行“同一健康”理念。
