Institut de Recerca i Tecnologia Agroalimentàries, Centre for Research in Agricultural Genomics Consejo Superior de Investigaciones Científicas-Institut de Recerca i Tecnologia Agroalimentàries-Universitat Autònoma de Barcelona-Universitat de Barcelona, 08193 Barcelona, Spain.
Proc Natl Acad Sci U S A. 2012 Jul 17;109(29):11872-7. doi: 10.1073/pnas.1205415109. Epub 2012 Jul 2.
We report the genome sequence of melon, an important horticultural crop worldwide. We assembled 375 Mb of the double-haploid line DHL92, representing 83.3% of the estimated melon genome. We predicted 27,427 protein-coding genes, which we analyzed by reconstructing 22,218 phylogenetic trees, allowing mapping of the orthology and paralogy relationships of sequenced plant genomes. We observed the absence of recent whole-genome duplications in the melon lineage since the ancient eudicot triplication, and our data suggest that transposon amplification may in part explain the increased size of the melon genome compared with the close relative cucumber. A low number of nucleotide-binding site-leucine-rich repeat disease resistance genes were annotated, suggesting the existence of specific defense mechanisms in this species. The DHL92 genome was compared with that of its parental lines allowing the quantification of sequence variability in the species. The use of the genome sequence in future investigations will facilitate the understanding of evolution of cucurbits and the improvement of breeding strategies.
我们报告了甜瓜的基因组序列，甜瓜是一种重要的世界性园艺作物。我们组装了双单倍体系 DHL92 的 375Mb 基因组序列，代表了甜瓜基因组估计大小的 83.3%。我们预测了 27427 个编码蛋白的基因，并通过构建 22218 个系统发育树来分析这些基因，从而可以对已测序植物基因组的同源和旁系关系进行映射。我们观察到，自古老的真双子叶植物三倍体化以来，甜瓜谱系中没有发生近期的全基因组加倍事件，我们的数据表明，转座子扩增可能部分解释了与近亲黄瓜相比，甜瓜基因组大小的增加。注释的核苷酸结合位点富含亮氨酸重复的抗病基因数量较少，这表明该物种存在特定的防御机制。将 DHL92 基因组与亲本系进行比较，可量化该物种的序列变异性。在未来的研究中使用基因组序列将有助于理解葫芦科的进化，并改进其育种策略。
