Delling Uta, Lindner Katrin, Ribitsch Iris, Jülke Henriette, Brehm Walter
Large Animal Clinic for Surgery, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 21, 04103 Leipzig, Germany.
Can J Vet Res. 2012 Jan;76(1):52-6.
The objective of this study was to compare bone marrow (BM) aspirates from the sternum and the tuber coxae of middle-aged horses. Bone marrow was obtained from the sternum and both tubera coxae of 12 healthy, 13-year-old geldings. Two different puncture techniques were used for the tuber coxae. The 2 syringes used for sternal sampling were evaluated separately. The mononuclear cell (MNC) fraction of the BM was isolated and the mesenchymal stem cells (MSCs) were culture-expanded. At the sternum, BM aspiration was always possible. Bone marrow aspiration at the tuber coxae required straight and deep needle penetration combined with high negative pressure. With this technique a median sample amount of 11.0 mL with large individual variation was obtained. A median of 3.06 × 10(6) MNC/mL BM (1st syringe) and 2.46 × 10(6) MNC/mL BM (2nd syringe) was isolated from sternal samples. In contrast, the tuber coxae yielded a median of 0.27 × 10(6) MNC/mL BM. The first passage yielded a median of 2.19 × 10(6) MSC (1st syringe) and 1.13 × 10(6) MSC (2nd syringe) from sternal samples, compared to a significantly lower median number of MSC from tuber coxae BM (0.06 × 10(6) MSC). The number of MNC and MSC obtainable from the BM aspirates taken from the tuber coxae is significantly lower than that obtained from the sternal BM aspirates. Autologous BM for the equine athlete is particularly clinically relevant at an advanced age. Based on our findings, the tuber coxae cannot be recommended for BM aspiration in middle-aged horses.
本研究的目的是比较中年马匹胸骨和髋结节处的骨髓穿刺物。从12匹健康的13岁雄性马的胸骨和双侧髋结节获取骨髓。髋结节采用两种不同的穿刺技术。用于胸骨采样的2支注射器分别进行评估。分离骨髓的单核细胞（MNC）组分并对间充质干细胞（MSC）进行培养扩增。在胸骨处，总是能够成功进行骨髓穿刺。髋结节处的骨髓穿刺需要直针且深穿刺并结合高负压。采用该技术获得的样本量中位数为11.0 mL，个体差异较大。从胸骨样本中分离出的MNC中位数为第1支注射器3.06×10⁶个MNC/mL骨髓和第2支注射器2.46×10⁶个MNC/mL骨髓。相比之下，髋结节处产生的MNC中位数为0.
