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外周血源性间充质干细胞具有免疫调节潜力，可用于马的治疗。

Peripheral blood-derived mesenchymal stem cells demonstrate immunomodulatory potential for therapeutic use in horses.

机构信息

Department of Ophthalmology and Visual Sciences, University of Alabama at Birmingham, Birmingham, Alabama, United States of America.

Department of Ophthalmology, Indiana University School of Medicine, Indianapolis, IN, United States of America.

出版信息

PLoS One. 2019 Mar 14;14(3):e0212642. doi: 10.1371/journal.pone.0212642. eCollection 2019.

DOI:10.1371/journal.pone.0212642
PMID:30870461
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6417789/
Abstract

Previously, we showed that mesenchymal stem cells (MSC) can be mobilized into peripheral blood using electroacupuncture (EA) at acupoints, LI-4, LI-11, GV-14, and GV-20. The purpose of this study was to determine whether EA-mobilized MSC could be harvested and expanded in vitro to be used as an autologous cell therapy in horses. Peripheral blood mononuclear cells (PBMC) isolated from young and aged lame horses (n = 29) showed a marked enrichment for MSCs. MSC were expanded in vitro (n = 25) and administered intravenously at a dose of 50 x 106 (n = 24). Treatment resulted in significant improvement in lameness as assessed by the American Association of Equine Practitioners (AAEP) lameness scale (n = 23). MSCs exhibited immunomodulatory function by inhibition of lymphocyte proliferation and induction of IL-10. Intradermal testing showed no immediate or delayed immune reactions to MSC (1 x 106 to 1 x 104). In this study, we demonstrated an efficient, safe and reproducible method to mobilize and expand, in vitro, MSCs in sufficiently high concentrations for therapeutic administration. We confirm the immunomodulatory function of these cells in vitro. This non-pharmacological and non-surgical strategy for stem cell harvest has a broad range of biomedical applications and represents an improved clinically translatable and economical cell source for humans.

摘要

先前，我们已经证明电针对穴位（LI-4、LI-11、GV-14 和 GV-20）的刺激可以将间充质干细胞（MSC）动员到外周血中。本研究旨在确定是否可以从年轻和年老的跛行马（n = 29）的外周血单核细胞（PBMC）中收获和体外扩增 EA 动员的 MSC，作为马的自体细胞治疗。分离的 MSC 在体外进行了扩增（n = 25），并以 50 x 106 的剂量静脉内给药（n = 24）。治疗后，美国兽医协会（AAEP）跛行评分（n = 23）评估跛行明显改善。MSC 通过抑制淋巴细胞增殖和诱导 IL-10 表现出免疫调节功能。皮内试验未显示对 MSC（1 x 106 至 1 x 104）的即刻或延迟免疫反应。在这项研究中，我们证明了一种高效、安全且可重复的方法，可在外周血中动员和扩增足够高浓度的 MSC 以进行治疗。我们在体外证实了这些细胞的免疫调节功能。这种非药理学和非手术的干细胞采集策略具有广泛的生物医学应用，并代表了一种改善的临床可转化和经济的人类细胞来源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db4f/6417789/304b8ef9719d/pone.0212642.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db4f/6417789/072b98be92ef/pone.0212642.g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db4f/6417789/e8a016ed3810/pone.0212642.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db4f/6417789/33eae2eb01d4/pone.0212642.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db4f/6417789/6b1f8b199126/pone.0212642.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db4f/6417789/304b8ef9719d/pone.0212642.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db4f/6417789/072b98be92ef/pone.0212642.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db4f/6417789/eaf2324b2179/pone.0212642.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db4f/6417789/d3db342033f2/pone.0212642.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db4f/6417789/e8a016ed3810/pone.0212642.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db4f/6417789/33eae2eb01d4/pone.0212642.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db4f/6417789/6b1f8b199126/pone.0212642.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db4f/6417789/304b8ef9719d/pone.0212642.g007.jpg

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