Gail Holmes Equine Orthopaedic Research Center, College of Veterinary Medicine and Biomedical Science, Colorado State University , Fort Collins, CO , USA.
Department of Comparative Pathobiology, Purdue University , West Lafayette, IN , USA.
Front Vet Sci. 2015 Dec 7;2:67. doi: 10.3389/fvets.2015.00067. eCollection 2015.
Diseases of the musculoskeletal system are a major cause of loss of use and retirement in sport horses. The use of bone marrow-derived mesenchymal stem cells (BMDMSCs) for healing of traumatized tissue has gained substantial favor in clinical settings and can assist healing and tissue regeneration in orthopedic injuries. There are two common sites of harvest of BMDMSCs, the sternum and the ilium. Our objective was to determine if any differences exist in BMDMSCs acquired from the sternum and the ilium. We compared the two harvest sites in their propensity to undergo multilineage differentiation, differences in cell surface markers, or gene transduction efficiencies. BMDMSCs were isolated and culture-expanded from 5 ml aspirates of bone marrow from sternum and ilium. The cells were then plated and cultured with appropriate differentiation medium to result in multi-lineage differentiation and cell characteristics were compared between sternal and ilial samples. Cell surface antibody expression of CD11a/18, CD34, CD44, and CD90 were evaluated using flow cytometry, and gene transduction efficiencies were evaluated using GFP scAAV. There were no statistically significant differences in cell characteristics between MSCs cultured from the sternum and the ilium under any circumstances.
肌肉骨骼系统疾病是导致运动马丧失用途和退役的主要原因。骨髓间充质干细胞（BMDMSCs）在创伤组织的修复中得到了广泛的应用，并能辅助骨科损伤的愈合和组织再生。BMDMSCs 的常见采集部位有胸骨和髂骨。我们的目的是确定从胸骨和髂骨获取的 BMDMSCs 是否存在差异。我们比较了这两个采集部位在多向分化潜能、细胞表面标志物差异或基因转导效率方面的差异。从胸骨和髂骨的骨髓抽吸物中分离和培养 BMDMSCs，然后将细胞接种并培养于适当的分化培养基中，以实现多向分化，并比较胸骨和髂骨样本之间的细胞特征。使用流式细胞术评估 CD11a/18、CD34、CD44 和 CD90 的细胞表面抗体表达，使用 GFP scAAV 评估基因转导效率。在任何情况下，从胸骨和髂骨培养的 MSC 之间的细胞特征均无统计学差异。
