中国大陆日本血吸虫中间宿主钉螺中15个新微卫星标记的分离与鉴定
Isolation and characterization of 15 new microsatellite markers in Oncomelania hupensis, the snail intermediate host of Schistosoma japonicum in mainland China.
作者信息
Zhang Li, Li Shizhu, Wang Qiang, Qian Yingjun, Liu Qin, Yang Pin, Zhou Xiaonong
机构信息
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China.
出版信息
Int J Mol Sci. 2012;13(5):5844-5850. doi: 10.3390/ijms13055844. Epub 2012 May 15.
Abstract
Oncomelania hupensis is the unique intermediate host of Schistosoma japonicum, which plays a key role during the transmission of schistosomiasis. It is mainly found in the Yangtze River valley and mountains or hills in southwest China. In this paper, we described 15 new microsatellite makers in O. hupensis. Polymorphism of each locus was assessed in 80 individuals from four wild populations (n = 20 per population). The number of alleles per locus ranged from 6 to 29, with an average of 15.8. The observed (H(O)) and expected (H(E)) heterozygosities varied from 0.397 to 0.851 and from 0.696 to 0.948, respectively. These microsatellite markers will be useful for population genetic studies and genome mapping in O. hupensis.
摘要
湖北钉螺是日本血吸虫唯一的中间宿主，在血吸虫病传播过程中起着关键作用。它主要分布在中国长江流域以及西南地区的山区或丘陵地带。在本文中，我们描述了湖北钉螺的15个新微卫星标记。对来自四个野生种群（每个种群n = 20）的80个个体评估了每个位点的多态性。每个位点的等位基因数量范围为6至29，平均为15.8。观察到的杂合度（H(O)）和预期杂合度（H(E)）分别在0.397至0.851和0.696至0.948之间变化。这些微卫星标记将有助于湖北钉螺的群体遗传学研究和基因组作图。
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