Seong Nak-Won, Oh Won-Jun, Kim Il-Soo, Kim Su-Jin, Seo Ji-Eun, Park Chang-Eon, Kim Da-Young, Ko Je-Won, Kim Jong-Choon
1Health Care Institute, Korea Testing and Research Institute, Hwasun, Jeonnam 58141 Republic of Korea.
2College of Veterinary Medicine (BK21 Plus Project Team), Chonnam National University, Gwangju, 61186 Republic of Korea.
Lab Anim Res. 2019 Jun 24;35:4. doi: 10.1186/s42826-019-0003-3. eCollection 2019.
Although leaves have been studied as a raw material for various cosmetic products, little is known about the anti-oxidant, anti-inflammatory, and anti-melanogenic activities of leaf ethanol extract (EJEE).
This study was conducted to evaluate the anti-oxidant, anti-inflammatory, and anti-melanogenic activities of EJEE using different in vitro models. In addition, we investigated the potential irritation of EJEE to skin and eye using animal alternative tests.
The total content of polyphenols, one of the active constituents of EJEE, was analyzed by high-performance liquid chromatography and found to contain 88.68 mg tannic acid equivalent/g. EJEE showed a concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging activity, and a superoxide dismutase-like activity. The anti-inflammatory effect of 0.5% (w/v) EJEE was demonstrated by a reduction in lipopolysaccharide-induced nitric oxide and tumor necrosis factor-alpha levels in RAW 264.7 cells. EJEE also significantly inhibited melanogenesis in melanocyte stimulating hormone-induced B16F1 cells. EJEE did not show any irritation in skin and eye in animal alternative test.
These results indicate that the EJEE possesses anti-oxidant, anti-inflammatory, and anti-melanogenic activities, while it did not induce toxicity or irritation in neither skin nor eye. Therefore, EJEE can be used as a cosmetic ingredient for skin improvement.
尽管树叶已被研究作为各种化妆品的原料，但关于树叶乙醇提取物（EJEE）的抗氧化、抗炎和抗黑色素生成活性知之甚少。
本研究旨在使用不同的体外模型评估EJEE的抗氧化、抗炎和抗黑色素生成活性。此外，我们使用动物替代试验研究了EJEE对皮肤和眼睛的潜在刺激性。
通过高效液相色谱法分析了EJEE的活性成分之一多酚的总含量，发现其含有88.68毫克单宁酸当量/克。EJEE表现出浓度依赖性的1,1-二苯基-2-苦基肼自由基清除活性、2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）自由基清除活性和超氧化物歧化酶样活性。0.5%（w/v）的EJEE通过降低RAW 264.7细胞中脂多糖诱导的一氧化氮和肿瘤坏死因子-α水平，证明了其抗炎作用。EJEE还显著抑制了促黑素细胞激素诱导的B16F1细胞中的黑色素生成。在动物替代试验中，EJEE在皮肤和眼睛上未表现出任何刺激性。
这些结果表明，EJEE具有抗氧化、抗炎和抗黑色素生成活性，同时在皮肤和眼睛中均未诱导毒性或刺激性。因此，EJEE可作为一种用于改善皮肤的化妆品成分。
