ProBioGen AG, Goethestr. 54, Berlin 13086, Germany.
Viruses. 2012 May;4(5):889-900. doi: 10.3390/v4050889. Epub 2012 May 23.
Fruit bats and insectivorous bats are believed to provide a natural reservoir for a wide variety of infectious diseases. Several lines of evidence, including the successful isolation of infectious viruses, indicate that Marburg virus and Ravn virus have found a major reservoir in colonies of the Egyptian rousette (Rousettus aegyptiacus). To facilitate molecular studies on virus-reservoir host interactions and isolation of viruses from environmental samples, we established cell lines from primary cells of this animal. The cell lines were given to several laboratories until we realized that a contamination with Vero cells in one of the cultures had occurred. Here we describe a general diagnostic procedure for identification of cross-species contamination with the focus on Vero and Rousettus cell lines, and summarize newly discovered properties of the cell lines that may pertain to pathogen discovery.
果蝠和食虫蝙蝠被认为是多种传染病的天然宿主。包括成功分离传染性病毒在内的几条证据表明，马尔堡病毒和拉文病毒在埃及果蝠（Rousettus aegyptiacus）的种群中找到了主要的宿主。为了促进病毒-宿主相互作用的分子研究以及从环境样本中分离病毒，我们从这种动物的原代细胞中建立了细胞系。这些细胞系被提供给了几个实验室，直到我们意识到其中一个培养物中发生了与非洲绿猴肾细胞（Vero）的交叉污染。在这里，我们描述了一种用于鉴定跨物种污染的通用诊断程序，重点是 Vero 和 Rousettus 细胞系，并总结了这些细胞系新发现的特性，这些特性可能与病原体的发现有关。
