Gokani Rina H, Lahiri Suman K, Santani Devdas D, Shah Mamta B
LM College of Pharmacy.
J Complement Integr Med. 2011 Jan;8. doi: 10.2202/1553-3840.1216.
Roots of Premna integrifolia Linn. Mant. (Verbanacea) are important rasayana (Adaptogenic) drugs and are considered to be useful in the treatment of variety of ailments. The present study was aimed at evaluating the for its anti-inflammatory and antioxidant activities. Pretreatment with a single dose of methanolic extract of P. integrifolia (PIM) (300 mg/kg b.w.) produced significant inhibition on carrageenan-induced rat hind paw edema, histamine induced wheal formation, and acetic acid-induced mouse vascular permeation. In a 7-day study, daily administration of PIM suppressed formalin induced paw edema and cotton pellet-induced rat granuloma formation. The extract also showed significant inhibition of cyclo-oxygenase (COX-I) activity on rat uterus and plasma membrane stabilization. Apart from this, the antioxidant activity (in vitro) of the extracts was evaluated using the anti radical, superoxide scavenging, erythrocyte membrane stability, anti lipid peroxidation, hydroxyl radical scavenging, nitric oxide scavenging and reducing power (ferric thiocynate method and β-carotene bleaching test) assays. PIM showed significant anti-oxidant activity. In addition, PIM (300mg/kg b.w.) produced no observable sub acute toxicity in mice with in 15 days. The results scientifically demonstrated the antiinflammatory activity of P. integrifolia roots in various experimental models probably through their antihistaminic, antikinin, COX-inhibitory and antioxidant action. This therefore justifies the folkloric use of the plant.
风车草（马鞭草科）的根是重要的滋补药（适应原性药物），被认为可用于治疗多种疾病。本研究旨在评估其抗炎和抗氧化活性。用单剂量的风车草甲醇提取物（PIM）（300毫克/千克体重）预处理，对角叉菜胶诱导的大鼠后爪水肿、组胺诱导的风团形成和乙酸诱导的小鼠血管通透性有显著抑制作用。在一项为期7天的研究中，每天给予PIM可抑制福尔马林诱导的爪水肿和棉球诱导的大鼠肉芽肿形成。该提取物还显示出对大鼠子宫环氧化酶（COX-I）活性的显著抑制作用以及质膜稳定作用。除此之外，使用抗自由基、超氧化物清除、红细胞膜稳定性、抗脂质过氧化、羟自由基清除、一氧化氮清除和还原能力（硫氰酸铁法和β-胡萝卜素漂白试验）测定法评估了提取物的抗氧化活性（体外）。PIM显示出显著的抗氧化活性。此外，PIM（300毫克/千克体重）在15天内对小鼠未产生可观察到的亚急性毒性。结果科学地证明了风车草根在各种实验模型中的抗炎活性，可能是通过其抗组胺、抗激肽、COX抑制和抗氧化作用。因此，这证明了该植物在民间的用途是合理的。
