Singh Chandrashekhar, Tiwari Kavindra Nath, Kumar Pradeep, Kumar Anil, Dixit Jyoti, Saini Rajesh, Mishra Sunil Kumar
Department of Botany, MMV, Banaras Hindu University, Varanasi, Uttar Pradesh, 221005, India.
Department of Pharmaceutical Engineering & Technology, Indian Institute of Technology, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India.
Toxicol Rep. 2021 Jan 8;8:196-205. doi: 10.1016/j.toxrep.2021.01.004. eCollection 2021.
L. (Lamiaceae) is widely used in herbal formulation "Dashmoolarishta" which is useful in postnatal care. Ethyl acetate extract obtained from the leaves was evaluated for phenolic content and its antioxidant activity. Acute and subacute toxicity of the extract was studied in mice of both sexes to get an idea about LD value and assessed its safety profile before its application as a protective agent against different toxicities induced by xenobiotics. Phenol enriched extract (phenol content is 63.10 ± 1.26 mg/g of gallic acid equivalent and flavonoid content 75.33 ± 0.23 mg/g of rutin equivalent) showed good antioxidant activity. In acute toxicity studies it was observed that single different doses (300-5000 mg/kg b.wt.) of extract did not show any mortality of mice. Thus the LD of the extract was determined, and it was higher than 5000 mg/kg. There was no major change in behavioral and general appearance of mice. External morphology of liver, kidneys, lungs, spleen and heart did not show any effect of treatment. In subacute toxicity no statistically significant change in body weight, relative organ weight, food intake and water uptake, hematological, biochemical parameters were reported after comparison with control. Extract did not show significant effect in the level of antioxidant enzymes in the liver of mice of treated groups. No histopathological changes were observed in liver and kidney tissues. Thus, extract did not show any sign of toxic effects, when administered orally to male and female mice at dose level up to 1000 mg/kg. So, it can be utilized as protective agent against toxicity produced by different xenobiotics.
唇形科植物在草药配方“Dashmoolarishta”中广泛应用，该配方对产后护理有益。对从叶片中获得的乙酸乙酯提取物进行了酚类含量及其抗氧化活性的评估。在雌雄小鼠中研究了该提取物的急性和亚急性毒性，以了解其半数致死量（LD）值，并在将其用作针对异生物素诱导的不同毒性的保护剂之前评估其安全性。富含酚类的提取物（酚含量为63.10±1.26毫克/克没食子酸当量，黄酮含量为75.33±0.23毫克/克芦丁当量）表现出良好的抗氧化活性。在急性毒性研究中观察到，不同的单一剂量（300 - 5000毫克/千克体重）提取物未导致小鼠死亡。因此确定了该提取物的LD，其高于5000毫克/千克。小鼠的行为和一般外观没有重大变化。肝脏、肾脏、肺、脾脏和心脏的外部形态未显示出任何治疗效果。在亚急性毒性研究中，与对照组相比，未报告体重、相对器官重量、食物摄入量和饮水量、血液学、生化参数有统计学显著变化。提取物在处理组小鼠肝脏中的抗氧化酶水平上未显示出显著影响。在肝脏和肾脏组织中未观察到组织病理学变化。因此，当以高达1000毫克/千克的剂量口服给予雄性和雌性小鼠时，提取物未显示出任何毒性作用迹象。所以，它可以用作针对不同异生物素产生的毒性的保护剂。
