Navayath Sushma, Thiyagarajan Devasena
Vels University.
J Complement Integr Med. 2011 Jan;8. doi: 10.2202/1553-3840.1436.
Erythrocytes are excellent model to study the xenobiotic induced oxidative changes. Pyrethroid pesticides are increasingly being used in insecticidal preparations from the simple mosquito coils to house hold aerosols to sophisticated ultra low volume foggers and sprays. Cypermethrin a Type II pyrethroid pesticide is used widely in pest control. Fenugreek is a potent antioxidant. We have evaluated the potential of aqueous extract of germinated fenugreek seeds in counteracting cypermethrin induced oxidative changes in erythrocytes of male Wistar rats. Male Wistar rats were treated with 1/10 LD50 (25mg/kg body weight) of cypermethrin and 10 percent aqueous extract of germinated fenugreek for 60 days. Cypermethrin treatment caused significant decrease in non enzymatic antioxidants, glutathione (GSH), vitamin E, vitamin C, increased methemoglobin formation in erythrocytes and increased their mechanical fragility. Treatment with fenugreek reversed the cypermethrin induced oxidative changes in erythrocytes and restored all the parameters to near normal levels. The overall results reveal the ameliorating effect of aqueous extract of germinated fenugreek on cypermethrin induced toxicity in erythrocytes.
红细胞是研究外源性物质诱导氧化变化的优秀模型。拟除虫菊酯类农药越来越多地用于杀虫制剂中，从简单的蚊香到家用气雾剂，再到复杂的超低容量喷雾器和喷雾剂。氯氰菊酯是一种II型拟除虫菊酯类农药，广泛用于害虫防治。胡芦巴是一种有效的抗氧化剂。我们评估了发芽胡芦巴种子水提取物对抗氯氰菊酯诱导的雄性Wistar大鼠红细胞氧化变化的潜力。雄性Wistar大鼠用1/10 LD50（25mg/kg体重）的氯氰菊酯和10%发芽胡芦巴水提取物处理60天。氯氰菊酯处理导致非酶抗氧化剂、谷胱甘肽（GSH）、维生素E、维生素C显著降低，红细胞中高铁血红蛋白形成增加，机械脆性增加。用胡芦巴处理可逆转氯氰菊酯诱导的红细胞氧化变化，并使所有参数恢复到接近正常水平。总体结果揭示了发芽胡芦巴水提取物对氯氰菊酯诱导的红细胞毒性的改善作用。
