Sankar Palanisamy, Telang Avinash G, Manimaran Ayyasamy
Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar 243122, Bareilly, Uttar Pradesh, India.
Exp Toxicol Pathol. 2012 Jul;64(5):487-93. doi: 10.1016/j.etp.2010.11.003. Epub 2010 Dec 4.
The aim of present study was to investigate the protective effect of curcumin on cypermethrin-induced changes in blood biochemical markers and tissue antioxidant enzyme in rats. Rats were divided into six groups of six each: group I used as control and II and III groups were used as vehicle control. While, groups IV, V and VI were orally treated with curcumin (100 mg/kg body weight), cypermethrin (25 mg/kg body weight) and cypermethrin plus curcumin, respectively for 28 days. Serum biochemical markers were measured in the serum, and the levels of lipid peroxidation and antioxidant enzyme activity were determined in the liver, kidney and brain. Cypermethrin administration caused elevated level of blood biochemical markers in serum and lipid peroxidation in liver, kidney and brain. While the activities of non-enzymatic and enzymatic antioxidants levels were decreased except superoxide dismutase in liver, kidney and brain tissues. The presence of curcumin with cypermethrin significantly decreased the blood biochemical markers and lipid peroxidation but significantly increased the reduced glutathione, catalase and glutathione peroxidase level and preserved the normal histological architecture of the liver, kidney and brain. Our results indicate that curcumin can be potent protective agent against cypermethrin-induced biochemical alterations and oxidative damage in rats.
本研究的目的是探讨姜黄素对氯氰菊酯诱导的大鼠血液生化指标变化及组织抗氧化酶的保护作用。将大鼠分为六组，每组六只：第一组作为对照组，第二组和第三组作为溶剂对照组。而第四组、第五组和第六组分别口服姜黄素（100毫克/千克体重）、氯氰菊酯（25毫克/千克体重）和氯氰菊酯加姜黄素，持续28天。测定血清中的血清生化指标，并测定肝脏、肾脏和大脑中的脂质过氧化水平和抗氧化酶活性。给予氯氰菊酯导致血清中血液生化指标水平升高以及肝脏、肾脏和大脑中的脂质过氧化。而肝脏、肾脏和脑组织中非酶类和酶类抗氧化剂水平的活性降低，但肝脏中超氧化物歧化酶除外。姜黄素与氯氰菊酯同时存在时，可显著降低血液生化指标和脂质过氧化，但显著提高还原型谷胱甘肽、过氧化氢酶和谷胱甘肽过氧化物酶水平，并维持肝脏、肾脏和大脑的正常组织结构。我们的结果表明，姜黄素可以作为一种有效的保护剂，对抗氯氰菊酯诱导的大鼠生化改变和氧化损伤。
