基于串联重复多态性的脓肿分枝杆菌分子分型。
Molecular typing of Mycobacterium abscessus based on tandem-repeat polymorphism.
机构信息
Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia.
出版信息
J Clin Microbiol. 2012 Sep;50(9):3084-8. doi: 10.1128/JCM.00753-12. Epub 2012 Jul 3.
Abstract
A variable-number tandem-repeat (VNTR) typing assay for the differentiation of Mycobacterium abscessus strains was developed. This assay showed complete reproducibility, locus stability, and a discriminatory power (Hunter-Gaston discriminatory index [HGDI] of 0.9563) that is superior to that of multilocus sequencing. It is a promising tool for the investigation of Mycobacterium abscessus epidemiology and nosocomial outbreaks.
摘要
开发了一种用于区分脓肿分枝杆菌菌株的可变数串联重复（VNTR）分型检测方法。该检测方法显示出完全的可重复性、基因座稳定性和较高的区分能力（汉纳-加斯顿鉴别指数[HGDI]为 0.9563），优于多位点测序。它是一种很有前途的研究脓肿分枝杆菌流行病学和医院感染暴发的工具。
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