新型基于 PCR 的可变数串联重复序列分析对脓肿分枝杆菌复合体分离株进行种水平的高效鉴定。
Efficient differentiation of Mycobacterium abscessus complex isolates to the species level by a novel PCR-based variable-number tandem-repeat assay.
机构信息
Department of Laboratory Medicine, School of Medicine, Pusan National University, Yangsan, Gyeongsangnam-do 626-770, South Korea.
出版信息
J Clin Microbiol. 2011 Mar;49(3):1107-9. doi: 10.1128/JCM.02318-10. Epub 2010 Dec 22.
Abstract
A novel duplex PCR method based on variable-number tandem-repeat targets to discriminate among Mycobacterium abscessus complex isolates was developed and evaluated in 85 clinical isolates. The assay accuracy was confirmed by a multiple-target sequence analysis. The duplex PCR assay is a one-step, reliable, and accurate assay for discriminating M. abscessus species.
摘要
一种基于可变数目串联重复目标的新型双重 PCR 方法被开发出来，并在 85 株临床分离株中进行了评估。该方法的准确性通过多位点序列分析得到了确认。该双重 PCR 检测方法是一种一步法、可靠且准确的区分脓肿分枝杆菌种的方法。
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