Gaiba Silvana, França Lucimar Pereira de, França Jerônimo Pereira de, Ferreira Lydia Masako
Plastic Surgery Division, Department of Surgery, UNIFESP, Sao Paulo-SP, Brazil.
Acta Cir Bras. 2012 Jul;27(7):471-6. doi: 10.1590/s0102-86502012000700007.
There is a growing scientific interest in the plasticity and therapeutic potential of adipose-derived stem cells (ASCs), which are multipotent and abundant in adipose tissue and can differentiate in vitro into multiple lineages, including adipocytes, chondrocytes, osteoblasts, neural cells, endothelial cells and cardiomyocytes. The aim of this study was to isolate, cultivate and identify ASCs.
Human adipose precursor cells were obtained from subcutaneous abdominal tissue. Recently dispersed cells were separated by density centrifugation gradient, cultured and then analyzed.
Human ASCs were able to replicate in our culture conditions. The cells maintained their phenotypes throughout the studied period on different passages confirming they suitability for in vitro cultivation. We also induced their adipogenic, osteogenic and chondrogenic differentiation, verifying their mesenchymal stem cells potentiality in vitro. Flow cytometry results showed that these cells expressed CD73, CD90 and CD105, (mesenchymal stem-cells markers), contrasting with the lack of expression of CD16, CD34 and CD45 (hematopoietic cells markers).
It was possible to isolate human adipose-derived stem cells by in vitro cultivation without adipogenic induction, maintaining their functional integrity and high proliferation levels. The cells demonstrated adipogenic, osteogenic and chondrogenic differentiation potential in vitro.
脂肪来源干细胞（ASCs）具有可塑性和治疗潜力，这一领域正引发越来越多的科学关注。ASCs具有多能性，在脂肪组织中含量丰富，能够在体外分化为多种细胞谱系，包括脂肪细胞、软骨细胞、成骨细胞、神经细胞、内皮细胞和心肌细胞。本研究旨在分离、培养和鉴定ASCs。
从腹部皮下组织获取人脂肪前体细胞。通过密度梯度离心分离近期分散的细胞，进行培养并随后分析。
人ASCs能够在我们的培养条件下复制。在不同传代的整个研究期间，细胞保持其表型，证实它们适合体外培养。我们还诱导了它们的成脂、成骨和软骨分化，验证了它们在体外的间充质干细胞潜能。流式细胞术结果显示，这些细胞表达CD73、CD90和CD105（间充质干细胞标志物），与缺乏CD16、CD34和CD45（造血细胞标志物）的表达形成对比。
通过体外培养可以分离出人脂肪来源干细胞，无需成脂诱导，同时保持其功能完整性和高增殖水平。这些细胞在体外表现出成脂、成骨和软骨分化潜能。
