Brocco Marcos Célio, Paulo Danilo Nagib Salomão, Almeida Carlos Eduardo David de, Carraretto Antonio Roberto, Cabral Sigmar Aurea, Silveira Amanda Cardoso de Oliveira, Gomez Renato Santiago, Baptista João Florêncio de Abreu
Division of Anesthesiology, Department of Surgery, Espirito Santo Federal University, Brazil.
Acta Cir Bras. 2012 Jul;27(7):494-8. doi: 10.1590/s0102-86502012000700011.
The objective of this study was to assess the cytokine serum levels of IL-6 and TNF-α in rats subjected to fecal peritonitis and treated with peritoneal lavage with 0.2% ropivacaine by peritoneal lavage.
We subjected 16 Wistar rats to laparotomy 6 hours after the induction of fecal peritonitis with autogenous stool and subsequently divided the rats randomly into 4 groups: I-control, no treatment; II- drying of the abdominal cavity; III- lavage of the abdominal cavity with 3 mL of 0.9% normal saline and drying; IV- lavage of the abdominal cavity with 3 mL of 0.2% ropivacaine and drying. Six hours following the laparotomy, the animals underwent cardiac puncture, and 1 mL of blood was collected for cytokine assessment before the animals were euthanized.
The lavage with ropivacaine resulted in smaller TNF-α levels compared with those observed in the other treatment groups (p <0.05). Regarding IL-6, the ropivacaine group showed lower cytokine levels than those observed in groups I and II, but there was no significant difference (p> 0.05) between groups III and IV.
Peritoneal lavage with 0.2% ropivacaine was shown to reduce plasma levels of IL-6 and TNF-α in the treatment of fecal peritonitis in rats.
本研究的目的是评估经自体粪便诱导发生粪性腹膜炎并接受0.2%罗哌卡因腹腔灌洗治疗的大鼠血清中白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的细胞因子水平。
对16只Wistar大鼠在经自体粪便诱导发生粪性腹膜炎6小时后进行剖腹手术，随后将大鼠随机分为4组：I组-对照组，不进行治疗；II组-腹腔干燥；III组-用3 mL 0.9%生理盐水进行腹腔灌洗并干燥；IV组-用3 mL 0.2%罗哌卡因进行腹腔灌洗并干燥。剖腹手术后6小时，对动物进行心脏穿刺，在动物安乐死之前采集1 mL血液用于细胞因子评估。
与其他治疗组相比，罗哌卡因灌洗导致的TNF-α水平更低（p<0.05）。关于IL-6，罗哌卡因组的细胞因子水平低于I组和II组，但III组和IV组之间无显著差异（p>0.05）。
在大鼠粪性腹膜炎治疗中, 0.2%罗哌卡因腹腔灌洗可降低血浆IL-6和TNF-α水平。
