Unitat de Recerca en Lipids i Arteriosclerosi, CIBERDEM, Hospital Universitari Sant Joan, IISPV, Facultat de Medicina i Ciencies de la Salut, Universitat Rovira i Virgili, Reus, Spain.
Mol Nutr Food Res. 2012 Jul;56(7):1025-33. doi: 10.1002/mnfr.201100676.
Polyphenols (ingested via food items) can decrease DNA, and oxidative damage of proteins and lipids. However, polyphenol effects in healthy populations have not been well defined. The aim of this study was to assess the relationship between urinary total polyphenol excretion (TPE), a biomarker of total polyphenol intake (TPI), polyphenol-rich foods, and oxidative stress biomarkers in healthy adults of different ages participating in the cross-sectional PAScual MEDicina study.
Urinary TPE was determined by Folin-Ciocalteau method in spot urine samples of 81 participants (46 women), classified into three age groups: 18 to 39, 40 to 54, and 55 to 72 years of age. TPI was quantified from 3-day dietary records using the Phenol-Explorer database. Urinary TPE increased with age (p < 0.001). Urinary TPE was inversely associated with urinary 8-hydroxydeoxyguanosine (8-OHdG; p<0.001) and erythrocyte-oxidized glutathione concentrations (p < 0.05). A negative association between urinary 8-OHdG and daily intake of polyphenols from vegetables and fermented beverages such as red wine was observed.
Urinary TPE increased with age and may reflect attenuation of oxidative damage. These results could explain the beneficial effects in healthy individuals of a diet rich in vegetables and moderate red wine; food items typical of the Mediterranean diet.
多酚（通过食物摄入）可以减少 DNA 以及蛋白质和脂质的氧化损伤。然而，健康人群中的多酚作用尚未得到很好的定义。本研究旨在评估尿总多酚排泄量（TPE）与总多酚摄入量（TPI）、富含多酚的食物以及不同年龄段健康成年人氧化应激生物标志物之间的关系，这些成年人参与了横断面 PAScual MEDicina 研究。
使用 Folin-Ciocalteau 法在 81 名参与者（46 名女性）的随机尿样中测定尿 TPE，参与者被分为三个年龄组：18 至 39 岁、40 至 54 岁和 55 至 72 岁。使用 Phenol-Explorer 数据库从 3 天的饮食记录中定量 TPI。尿 TPE 随年龄增长而增加（p<0.001）。尿 TPE 与尿 8-羟基脱氧鸟苷（8-OHdG；p<0.001）和红细胞氧化型谷胱甘肽浓度呈负相关（p<0.05）。观察到尿 8-OHdG 与蔬菜和发酵饮料（如红酒）中多酚的每日摄入量呈负相关。
尿 TPE 随年龄增长而增加，可能反映了氧化损伤的减弱。这些结果可以解释富含蔬菜和适量红酒的饮食对健康个体的有益影响；这些食物是地中海饮食的典型代表。
