Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai 201203, China.
Inflammation. 2012 Oct;35(5):1715-22. doi: 10.1007/s10753-012-9489-7.
Radix Bupleuri is a traditional Chinese herb frequently used in the prescriptions for the treatment of inflammatory diseases. This study was to determine whether the crude polysaccharides isolated from the roots of Bupleurum smithii var. parvifolium (BPs) had beneficial effects on a "two-hit" acute lung injury model in rats. A two-hit lung injury model characterized by hemorrhagic shock followed by reperfusion and intratracheal lipopolysaccharide (1 mg kg(-1)) administration was established in Wistar rats. Drugs and saline were administered 30 min after the start of resuscitation. The two-hit acute lung injury model was successfully established. After oral administration, all BPs groups ameliorated pathological injury with lessened complement C3c deposit in lung. BPs 10 and 20 mg kg(-1) diminished the ratio of wet-to-dry weight. In bronchoalveolar lavage (BAL) fluids, the protein concentration, the leukocytes counts, and the lung myeloperoxidase were significantly reduced. BPs also mediated the decreases in interleukin-6 and tumor necrosis factor-α in BAL fluids and in sera. Furthermore, BPs 20 mg kg(-1) decreased the total complement hemolytic activity. BPs had beneficial effects on two-hit acute lung injury. The mechanisms of BPs on inflammatory disease might relate to its inhibitory effect on increased production of proinflammatory mediators and on overactivation of complement.
柴胡是一种常用于治疗炎症性疾病的中药。本研究旨在确定从狭叶柴胡（Bupleurum smithii var. parvifolium）根中分离得到的粗多糖（BPs）是否对大鼠“双打击”急性肺损伤模型具有有益作用。采用失血性休克后继发再灌注和气管内给予脂多糖（1mg/kg）的方法建立了一种以出血性休克后继发再灌注和气管内给予脂多糖（1mg/kg）为特征的“双打击”急性肺损伤模型。在复苏开始后 30min 给予药物和生理盐水。成功建立了“双打击”急性肺损伤模型。口服 BPs 后，所有 BPs 组均改善了肺组织的病理损伤，减少了肺内补体 C3c 的沉积。BPs 10 和 20mg/kg 降低了肺湿重/干重比。支气管肺泡灌洗液（BAL）中的蛋白浓度、白细胞计数和肺髓过氧化物酶明显降低。BPs 还介导了 BAL 液和血清中白细胞介素-6 和肿瘤坏死因子-α的减少。此外，BPs 20mg/kg 降低了总补体溶血活性。BPs 对“双打击”急性肺损伤具有有益作用。BPs 对炎症性疾病的作用机制可能与其抑制促炎介质产生增加和补体过度激活有关。
