Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai, China.
Phytomedicine. 2012 Jan 15;19(2):130-7. doi: 10.1016/j.phymed.2011.08.057. Epub 2011 Nov 22.
Bupleurum chinense DC had hepato-protective, anti-inflammatory, antipyretic, analgesic, and immunomodulatory effect in traditional Chinese medicine. This study was to determine whether the crude polysaccharides isolated from the roots of Bupleurum chinense DC (BCPs) attenuated lipopolysaccharide (LPS)-induced acute lung injury in mice. Mice were challenged with LPS intratracheally 2h before BCPs (20, 40 and 80 mg/kg) administration. The bronchoalveolar lavage fluid (BALF) was collected 24h after LPS challenge. Treatment with BCPs reduced lung wet-to-dry weight ratio. The elevated number of total cells and protein concentration in BALF was reduced. The increased level of myeloperoxidase (MPO), tumor necrosis factor-α (TNF-α) in BALF, and serum nitric oxide (NO) were also inhibited. BCPs significantly attenuated lung injury with improved lung morphology and reduced complement deposition. These results suggested that the effect of BCPs against ALI might be related with its inhibitory effect on excessive activation of complement and on the production of proinflammatory mediators.
柴胡具有保肝、抗炎、解热、镇痛和免疫调节作用。本研究旨在探讨柴胡根粗多糖（BCPs）是否能减轻脂多糖（LPS）诱导的小鼠急性肺损伤。小鼠在给予 BCPs（20、40 和 80mg/kg）前 2 小时经气管内滴注 LPS 造模。LPS 攻击 24 小时后收集支气管肺泡灌洗液（BALF）。BCPs 治疗可降低肺湿重/干重比，减少 BALF 中总细胞和蛋白浓度的增加，抑制髓过氧化物酶（MPO）、肿瘤坏死因子-α（TNF-α）在 BALF 和血清中的水平升高。BCPs 还能显著减轻肺损伤，改善肺形态，减少补体沉积。这些结果表明，BCPs 对 ALI 的作用可能与其抑制补体过度激活和促炎介质产生有关。
