ECERF W6-019, Department of Biomedical Engineering, University of Alberta, Edmonton, AB T6G 2V4, Canada.
Biotechnol Lett. 2012 Oct;34(10):1965-73. doi: 10.1007/s10529-012-0984-6. Epub 2012 Jul 5.
Low-intensity pulsed ultrasound (LIPUS) stimulated the viability, proliferation and differentiation of hematopoietic stem/progenitor cells (HSPC) from fresh and cryopreserved peripheral blood leukapheresis product, as well as cord blood when applied for 10 min each day for 4 days. Cell viability, proliferation and differentiation were assessed on day 5 by viable cell counting, MTS proliferation assay, flow cytometry, and colony-forming unit assay. LIPUS stimulation: (i) enhanced the proliferation of fresh HSPC and maintained the viability of cryopreserved HSPC in vitro; (ii) did not affect the percentage of CD34(+) and CD14(+) cells; and (iii) enhanced burst-forming unit-erythroid colony formation. Hence, we suggest that this novel LIPUS stimulation approach might enhance the efficacy of clinical transplantation and cellular therapies using HSPC.
低强度脉冲超声（LIPUS）刺激新鲜和冷冻保存的外周血白细胞分离产物以及脐血中的造血干细胞/祖细胞（HSPC）的活力、增殖和分化，每天应用 10 分钟，持续 4 天。在第 5 天通过活细胞计数、MTS 增殖测定、流式细胞术和集落形成单位测定评估细胞活力、增殖和分化。LIPUS 刺激：（i）增强新鲜 HSPC 的增殖并维持冷冻保存 HSPC 的活力；（ii）不影响 CD34(+)和 CD14(+)细胞的百分比；（iii）增强红细胞集落形成单位的形成。因此，我们认为这种新的 LIPUS 刺激方法可能会增强使用 HSPC 的临床移植和细胞治疗的疗效。
