Tang Jin, Feng Mingxuan, Wang Dong, Zhang Liang, Yang Ke
Pediatric Research Institute, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Engineering Research Center of Stem Cell Therapy, Chongqing 400014, China.
Department of Ultrasound, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Genes Dis. 2023 Sep 15;11(5):101112. doi: 10.1016/j.gendis.2023.101112. eCollection 2024 Sep.
Recent advancements in biomedical research have underscored the importance of noninvasive cellular manipulation techniques. Sonogenetics, a method that uses genetic engineering to produce ultrasound-sensitive proteins in target cells, is gaining prominence along with optogenetics, electrogenetics, and magnetogenetics. Upon stimulation with ultrasound, these proteins trigger a cascade of cellular activities and functions. Unlike traditional ultrasound modalities, sonogenetics offers enhanced spatial selectivity, improving precision and safety in disease treatment. This technology broadens the scope of non-surgical interventions across a wide range of clinical research and therapeutic applications, including neuromodulation, oncologic treatments, stem cell therapy, and beyond. Although current literature predominantly emphasizes ultrasonic neuromodulation, this review offers a comprehensive exploration of sonogenetics. We discuss ultrasound properties, the specific ultrasound-sensitive proteins employed in sonogenetics, and the technique's potential in managing conditions such as neurological disorders, cancer, and ophthalmic diseases, and in stem cell therapies. Our objective is to stimulate fresh perspectives for further research in this promising field.
生物医学研究的最新进展凸显了非侵入性细胞操作技术的重要性。声遗传学是一种利用基因工程在靶细胞中产生对超声敏感蛋白质的方法，它与光遗传学、电遗传学和磁遗传学一起日益受到关注。在用超声刺激时，这些蛋白质会引发一系列细胞活动和功能。与传统超声模式不同，声遗传学具有更高的空间选择性，提高了疾病治疗的精准性和安全性。这项技术拓宽了非手术干预的范围，涵盖广泛的临床研究和治疗应用，包括神经调节、肿瘤治疗、干细胞治疗等等。尽管当前文献主要强调超声神经调节，但本综述对声遗传学进行了全面探讨。我们讨论了超声特性、声遗传学中使用的特定超声敏感蛋白质，以及该技术在治疗神经系统疾病、癌症、眼科疾病等病症以及干细胞治疗中的潜力。我们的目标是激发在这个充满前景的领域进行进一步研究的新视角。
