Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Am J Trop Med Hyg. 2012 Jul;87(1):148-52. doi: 10.4269/ajtmh.2012.11-0583.
We evaluated the diagnostic accuracy of two broadly reactive rapid immunochromatographic tests (ICTs) for detection of IgM and IgG against Orientia tsutsugamushi by using archived acute-phase serum samples from 102 patients with laboratory-confirmed scrub typhus, and from 62 archived serum samples from patients with other causes of fever as a negative control. These ICTs were constructed by using a mixture of recombinant proteins: 1) C1, a chimeric protein containing epitopes of the 56-kD antigen from Karp and TA763 strains; 2) Ktr56; and 3) Gmr56. Sensitivities of the ICTs for detection of IgM and IgG were 90.2% (95% confidence interval [CI] = 84.4-96.0%) and 86.3% (95% CI = 80.9-93.8%), respectively. Specificities were 85.5% (95% CI = 73.9-92.2%) and 96.8% (95% CI = 90.3-100%), respectively. Both assays were more sensitive and specific than the standard immune immunofluorescence assay for the early diagnosis of scrub typhus.
我们使用 102 例经实验室确诊的恙虫病患者的急性相血清样本和 62 例来自其他发热原因患者的存档血清样本作为阴性对照，评估了两种广泛反应性的快速免疫层析检测（ICT）用于检测针对恙虫东方体的 IgM 和 IgG 的诊断准确性。这两种 ICT 是使用重组蛋白混合物构建的：1）C1，一种包含 Karp 和 TA763 株 56-kD 抗原表位的嵌合蛋白；2）Ktr56；和 3）Gmr56。ICT 检测 IgM 和 IgG 的敏感性分别为 90.2%（95%置信区间[CI] = 84.4-96.0%）和 86.3%（95% CI = 80.9-93.8%）。特异性分别为 85.5%（95% CI = 73.9-92.2%）和 96.8%（95% CI = 90.3-100%）。与标准免疫荧光免疫测定相比，这两种检测方法在恙虫病的早期诊断中均更敏感和特异。
