Bourthoumieu Sylvie, Magnaudeix Amandine, Terro Faraj, Leveque Philippe, Collin Alice, Yardin Catherine
Department of Histology and Cytogenetics, Limoges University Hospital, Limoges Cedex, France.
Bioelectromagnetics. 2013 Jan;34(1):52-60. doi: 10.1002/bem.21744. Epub 2012 Jul 5.
The potential effects of radiofrequency (RF) exposure on the genetic material of cells are very important to determine since genome instability of somatic cells may be linked to cancer development. In response to genetic damage, the p53 protein is activated and can induce cell cycle arrest allowing more time for DNA repair or elimination of damaged cells through apoptosis. The objective of this study was to investigate whether the exposure to RF electromagnetic fields, similar to those emitted by mobile phones of the second generation standard, Global System for Mobile Communications (GSM), may induce expression of the p53 protein and its activation by post-translational modifications in cultured human cells. The potential induction of p53 expression and activation by GSM-900 was investigated after in vitro exposure of human amniotic cells for 24 h to average specific absorption rates (SARs) of 0.25, 1, 2, and 4 W/kg in the temperature range of 36.3-39.7 °C. The exposures were carried out using a wire-patch cell (WPC) under strictly controlled conditions of temperature. Expression and activation of p53 by phosphorylation at serine 15 and 37 were studied using Western blot assay immediately after three independent exposures of cell cultures provided from three different donors. Bleomycin-exposed cells were used as a positive control. According to our results, no significant changes in the expression and activation of the p53 protein by phosphorylation at serine 15 and 37 were found following exposure to GSM-900 for 24 h at average SARs up to 4 W/kg in human embryonic cells.
确定射频（RF）暴露对细胞遗传物质的潜在影响非常重要，因为体细胞的基因组不稳定性可能与癌症发展有关。响应遗传损伤时，p53蛋白被激活，并可诱导细胞周期停滞，从而为DNA修复留出更多时间，或通过凋亡消除受损细胞。本研究的目的是调查暴露于类似于第二代标准移动电话全球移动通信系统（GSM）发出的射频电磁场，是否会在培养的人类细胞中诱导p53蛋白表达及其通过翻译后修饰的激活。在人羊膜细胞于36.3 - 39.7°C温度范围内体外暴露于0.25、1、2和4 W/kg的平均比吸收率（SAR）24小时后，研究了GSM - 900对p53表达和激活的潜在诱导作用。暴露是在严格控制温度的条件下使用线贴细胞（WPC）进行的。在对来自三个不同供体的细胞培养物进行三次独立暴露后，立即使用蛋白质印迹法研究丝氨酸15和37处磷酸化对p53的表达和激活情况。用博来霉素处理的细胞用作阳性对照。根据我们的结果，在人胚胎细胞中，暴露于平均SAR高达4 W/kg的GSM - 900 24小时后，未发现丝氨酸15和37处磷酸化对p53蛋白的表达和激活有显著变化。
