Kim Ju Hwan, Huh Yang Hoon, Kim Hak Rim
Department of Pharmacology, College of Medicine, Dankook University, Cheonan, Chungnam, Republic of Korea.
Nano-Bio EM Research Group, Korea Basic Science Institute, Gwahak-ro, Yuseong-gu, Daejeon, Republic of Korea.
PLoS One. 2016 Apr 13;11(4):e0153308. doi: 10.1371/journal.pone.0153308. eCollection 2016.
The extensive use of wireless mobile phones and associated communication devices has led to increasing public concern about potential biological health-related effects of the exposure to electromagnetic fields (EMFs). EMFs emitted by a mobile phone have been suggested to influence neuronal functions in the brain and affect behavior. However, the affects and phenotype of EMFs exposure are unclear. We applied radiofrequency (RF) of 835 MHz at a specific absorption rate (SAR) of 4.0 W/kg for 5 hours/day for 4 and 12 weeks to clarify the biological effects on mouse brain. Interestingly, microarray data indicated that a variety of autophagic related genes showed fold-change within small range after 835 MHz RF exposure. qRT-PCR revealed significant up-regulation of the autophagic genes Atg5, LC3A and LC3B in the striatum and hypothalamus after a 12-week RF. In parallel, protein expression of LC3B-II was also increased in both brain regions. Autophagosomes were observed in the striatum and hypothalamus of RF-exposed mice, based on neuronal transmission electron microscopy. Taken together, the results indicate that RF exposure of the brain can induce autophagy in neuronal tissues, providing insight into the protective mechanism or adaptation to RF stress.
无线移动电话及相关通信设备的广泛使用，已引发公众对暴露于电磁场（EMF）可能产生的与生物健康相关影响的日益关注。有研究表明，移动电话发出的EMF会影响大脑中的神经元功能并改变行为。然而，EMF暴露的影响和表型尚不清楚。我们以4.0 W/kg的比吸收率（SAR），每天5小时，分别照射4周和12周835 MHz的射频（RF），以阐明其对小鼠大脑的生物学效应。有趣的是，微阵列数据表明，835 MHz RF暴露后，多种自噬相关基因的倍数变化在小范围内。定量逆转录聚合酶链反应（qRT-PCR）显示，在12周的RF照射后，纹状体和下丘脑的自噬基因Atg5、LC3A和LC3B显著上调。同时，两个脑区的LC3B-II蛋白表达也增加。基于神经元透射电子显微镜观察，在RF暴露小鼠的纹状体和下丘脑中观察到自噬体。综上所述，结果表明大脑的RF暴露可诱导神经元组织中的自噬，为深入了解保护机制或对RF应激的适应性提供了线索。
