离子液体介导的用于选择性捕获和纯化柯里拉京的印迹整体柱的制备
Preparation of ionic liquid-mediated imprinted monolith for selective capture and purification of corilagin.
作者信息
Wang Chao, Li Xiang-Jie, Yang Jian, Zhao Yong-Xin, Liu Zhao-Sheng, Aisa Haji Akber
机构信息
Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, Xinjiang, China; State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China; University of Chinese Academy of Sciences, Beijing 100039, China.
Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, Xinjiang, China.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Jan 15;1041-1042:98-103. doi: 10.1016/j.jchromb.2016.12.023. Epub 2016 Dec 19.
A method for solid-phase extraction (SPE) of corilagin from natural plant extracts based on molecularly imprinted polymers (MIPs) was developed. For the preparation of corilagin-MIP monoliths, 4-vinylpyridine was used as functional monomer, and ethylene glycol dimethacrylate was used as cross-linking monomer, using a mixture of 1-butyl-3-methylimidazoliumtetrafluoroborate (ionic liquid)-N,N-dimethylformamide-dimethyl sulfoxide as a porogen. A morphological characteristic of the corilagin imprinted monolith was further studied by scanning electron microscopy and nitrogen sorption method. The greatest imprinting factor of COR was up to 9. The MIPs were used as solid-phase extraction (SPE) sorbents for purification of COR and the mean recoveries of corilagin was 78.0% with COR purity of 98.0% from the crude extract of phyllanthus urinaria L. The resulting COR-imprinted polymer also displayed the good performance of fragment imprinting polymer for gallic acid with the mean recoveries of 94.0% and purity of 99.7%.
开发了一种基于分子印迹聚合物（MIPs）从天然植物提取物中固相萃取（SPE）柯里拉京的方法。在制备柯里拉京-MIP整体柱时，以4-乙烯基吡啶为功能单体，乙二醇二甲基丙烯酸酯为交联单体，使用1-丁基-3-甲基咪唑四氟硼酸盐（离子液体）-N，N-二甲基甲酰胺-二甲基亚砜的混合物作为致孔剂。通过扫描电子显微镜和氮吸附法进一步研究了柯里拉京印迹整体柱的形态特征。COR的最大印迹因子高达9。MIPs用作固相萃取（SPE）吸附剂来纯化COR，从叶下珠粗提物中获得的柯里拉京平均回收率为78.0%，纯度为98.0%。所得的COR印迹聚合物对没食子酸也表现出良好的片段印迹聚合物性能，平均回收率为94.0%，纯度为99.7%。
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