King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Environ Monit Assess. 2013 Apr;185(4):2977-87. doi: 10.1007/s10661-012-2765-z. Epub 2012 Jul 7.
In present study, we measured the activities of some selected cytochrome P450 isozymes like ethoxyresorufin-o-deethylase (EROD), pentoxyresorufin-o-deethylase (PROD), and N-nitrosodimethylamine demethylase (NDMA-d) treating experimental rats with different environmental toxicants, namely trichloroethylene (TCE), heavy metal mixture, and ethyl alcohol individually or in combination and by exposing the Allium cepa bulbs to increasing concentration of TCE for 48 h. In animal system, NDMA-d activity displayed a remarkable amplification by 4.2-fold in the liver of alcohol ingested rats compared with control animals. TCE intake also resulted in a marked increase of around 3.8-fold and 1.2-fold in rats' NDMA-d activity in the liver and kidney, respectively. In onion bulbs, an amazing rise in the activity of test enzymes was recorded in a dose-dependent manner. Among the said enzymes, PROD showed maximum increase up to the extent of 22-fold in comparison with control at 20 ppm of TCE exposure, whereas NDMA-d and EROD showed 11- and 9-fold increase in enzymatic activity, respectively, compared with the control. Based on this study, we conclude and advocate that all the selected isozymes of CYP450 system, viz. PROD, NDMA-d, and EROD can act as potent biomarkers in plant system for assessing the TCE pollution.
在本研究中，我们测量了一些选定的细胞色素 P450 同工酶的活性，如乙氧基Resorufin-O-去乙基酶（EROD）、戊氧基Resorufin-O-去乙基酶（PROD）和 N-亚硝二甲胺脱甲基酶（NDMA-d），方法是用不同的环境毒物处理实验大鼠，即三氯乙烯（TCE）、重金属混合物和乙醇，单独或联合使用，并将 Allium cepa 鳞茎暴露于不断增加的 TCE 浓度下 48 小时。在动物系统中，与对照动物相比，摄入酒精的大鼠肝脏中的 NDMA-d 活性显著放大了 4.2 倍。TCE 的摄入也导致大鼠肝脏和肾脏中的 NDMA-d 活性分别显著增加了约 3.8 倍和 1.2 倍。在洋葱鳞茎中，测试酶的活性以剂量依赖性方式惊人地上升。在所述酶中，PROD 与对照相比显示出最大增加，达到 20ppm TCE 暴露时增加了 22 倍，而 NDMA-d 和 EROD 分别显示出 11 倍和 9 倍的酶活性增加。基于这项研究，我们得出结论并主张，所有选定的 CYP450 系统同工酶，即 PROD、NDMA-d 和 EROD，可以作为植物系统中评估 TCE 污染的有效生物标志物。
