Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Food Chem Toxicol. 2010 Jun;48(6):1581-6. doi: 10.1016/j.fct.2010.03.028. Epub 2010 Mar 21.
The use of food dyes is at least controversial because they are only of essential role. Moreover many of them have been related to health problems mainly in children that are considered a very vulnerable group. This study was carried out to investigate the effect of oral administration of Amaranth, Sunset Yellow and Curcumin for 4 weeks at doses of 47, 315 and 157.5 mg/kg b. wt. and after 2 weeks all animals were immunostimulated by intra peritoneal injection of sheep RBCs 10% (1 ml/rat). Body weight, relative body weight, total and differential leukocytes count, mononuclear cell count, delayed hypersensitivity, total protein and serum fractions were determined. Results revealed that oral administration of Amaranth, Sunset Yellow and Curcumin did not affect the body weight gain or the spleen weight. On the other hand Sunset Yellow and Curcumin significantly decreased the weight of thymus gland of the rats. Total leukocyte count were not affected while Amaranth and Curcumin-treated rats revealed a significant decrease in neutrophiles and monocytes and a compensatory increase in lymphocytes. Moreover, oral administration of Sunset Yellow revealed a significant decrease in monocyte percent. Amaranth, Sunset Yellow and Curcumin significantly decreased the delayed hyper sensitivity. Total serum protein, albumin, total globulin and albumin/globulin (A/G) ratio were not affected by administration of the colouring agents. Oral administration of Amaranth increases the density of albumin band. On the other hand oral administration of Curcumin decreases the density of the albumin band. Oral administration of any of the tested colouring agents did not change the density of globulin region as compared to control group. In conclusion we found that both synthetic (Amaranth and Sunset Yellow) and natural (Curcumin) colouring agents used at doses up to 10 times the acceptable daily intake exerted a depressing effect on the cellular but not humoral immune response.
食用色素的使用至少存在争议，因为它们只具有基本作用。此外，许多食用色素已被证明与健康问题有关，尤其是儿童，他们被认为是非常脆弱的群体。本研究旨在调查连续 4 周以 47、315 和 157.5mg/kg b.wt.剂量口服苋菜红、日落黄和姜黄素对大鼠的影响，2 周后所有动物均通过腹腔内注射 10%绵羊 RBC（1ml/大鼠）进行免疫刺激。测定体重、相对体重、总白细胞和分类白细胞计数、单核细胞计数、迟发型超敏反应、总蛋白和血清分数。结果表明，口服苋菜红、日落黄和姜黄素对体重增加或脾脏重量没有影响。另一方面，日落黄和姜黄素显著降低了大鼠胸腺的重量。总白细胞计数不受影响，而苋菜红和姜黄素处理的大鼠中性粒细胞和单核细胞显著减少，淋巴细胞代偿性增加。此外，日落黄组单核细胞百分比显著降低。苋菜红、日落黄和姜黄素显著降低迟发型超敏反应。总血清蛋白、白蛋白、总球蛋白和白蛋白/球蛋白（A/G）比值不受色素给药的影响。口服苋菜红增加了白蛋白带的密度。另一方面，口服姜黄素降低了白蛋白带的密度。与对照组相比，任何一种测试色素的口服给药都没有改变球蛋白区域的密度。总之，我们发现，即使在可接受的每日摄入量的 10 倍剂量下，合成（苋菜红和日落黄）和天然（姜黄素）色素均对细胞免疫而非体液免疫反应产生抑制作用。
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