Department of Nutrition, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
Horm Metab Res. 2012 Oct;44(11):814-8. doi: 10.1055/s-0032-1316350. Epub 2012 Jul 6.
The aim of the study was to verify if moderate physical training affects leptin content in visceral and subcutaneous adipose tissue of adult rats subjected to a low-protein diet during the perinatal period. Male Wistar rats were divided into 2 groups according to their mother's diet during gestation and lactation: control (17% casein, C, n=12) and low-protein (8% casein, LP, n=12). On postnatal day 60, half of each group was submitted to moderate physical training (8 wks, 5 d · wk - 1, 60 min · d - 1, at 70% of VO2max, T) or not. After the physical training period, visceral and subcutaneous adipose tissues were removed. Leptin content was evaluated by western blotting. Starting from the fifth week on, T pups showed a reduction in the body weight. Similarly, LP+T offspring showed a lower body weight starting from the sixth week on. Western blotting analysis showed that leptin content in the visceral tissue was higher in the LP rats (p<0.01) and it was reversed in LP+T. No difference was found in the subcutaneous tissue. Moderate physical training attenuated the effects of a perinatal low-protein diet on the leptin content in visceral adipose tissue in adult offspring.
这项研究的目的是验证在围产期给予低蛋白饮食的成年大鼠进行适度的体育锻炼是否会影响内脏和皮下脂肪组织中的瘦素含量。雄性 Wistar 大鼠根据其母亲在妊娠和哺乳期的饮食分为两组：对照组（17%酪蛋白，C，n=12）和低蛋白组（8%酪蛋白，LP，n=12）。在出生后第 60 天，每组的一半接受适度的体育锻炼（8 周，5 天/周，1 次/天，60 分钟/天，70%最大摄氧量，T）或不接受。体育锻炼期结束后，取出内脏和皮下脂肪组织。通过 Western blot 法评估瘦素含量。从第五周开始，T 组幼鼠体重减轻。类似地，从第六周开始，LP+T 后代的体重也较低。Western blot 分析显示，LP 组大鼠内脏组织中的瘦素含量较高（p<0.01），而 LP+T 组则有所逆转。在皮下组织中未发现差异。适度的体育锻炼减轻了围产期低蛋白饮食对成年后代内脏脂肪组织中瘦素含量的影响。
