3B's Research Group-Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, Portugal.
Stem Cell Rev Rep. 2012 Dec;8(4):1211-22. doi: 10.1007/s12015-012-9397-0.
Mesenchymal stem cells have a great potential for application in cell based therapies, such as tissue engineering. Adipose derived stem cells have shown the capacity to differentiate into several lineages, and have been isolated in many animal species. Dog is a very relevant animal model to study several human diseases and simultaneously an important subject in veterinary medicine. Thus, in this study we assessed the potential of canine adipose tissue derived stem cells (cASCs) to differentiate into the osteogenic and chondrogenic lineages by performing specific histological stainings, and studied the cell passaging effect on the cASCs stemness and osteogenic potential. We also evaluated the effect of the anatomical origin of the adipose tissue, namely from abdominal subcutaneous layer and from greater omentum. The stemness and osteogenic differentiation was followed by real time RT-PCR analysis of typical markers of mesenchymal stem cells (MSCs) and osteoblasts. The results obtained revealed that cASCs exhibit a progressively decreased expression of the MSCs markers along passages and also a decreased osteogenic differentiation potential. In the author's knowledge, this work presents the first data about the MSCs markers profile and osteogenic potential of cASCs along cellular expansion. Moreover, the obtained data showed that the anatomical origin of the adipose tissue has an evident effect in the differentiation potential of the ASCs. Due to the observed resemblances with the human ASCs, we conclude that canine ASCs can be used as a model cells in tissue engineering research envisioning human applications.
间充质干细胞在基于细胞的治疗方法（如组织工程学）中具有很大的应用潜力。脂肪来源的干细胞具有分化为多种谱系的能力，并已在许多动物物种中分离出来。狗是研究多种人类疾病的非常相关的动物模型，同时也是兽医领域的重要研究对象。因此，在这项研究中，我们通过进行特定的组织学染色来评估犬脂肪组织来源的干细胞（cASCs）向成骨和成软骨谱系分化的潜力，并研究细胞传代对 cASCs 干性和成骨潜能的影响。我们还评估了脂肪组织解剖学来源（即腹部皮下层和大网膜）对其的影响。通过实时 RT-PCR 分析间充质干细胞（MSCs）和成骨细胞的典型标志物来跟踪干细胞特性和成骨分化。结果表明，cASCs 在传代过程中 MSC 标志物的表达逐渐降低，成骨分化潜能也降低。据作者所知，这项工作首次提供了有关 cASCs 中 MSC 标志物谱和成骨潜能的细胞扩增数据。此外，获得的数据表明，脂肪组织的解剖学来源对 ASCs 的分化潜能有明显影响。由于观察到与人类 ASC 的相似性，我们得出结论，犬 ASC 可以作为组织工程研究中的模型细胞，用于设想的人类应用。
