Department of Biochemistry and Medical Genetics, School of Health Care, Medical University of Silesia, Poland.
Folia Neuropathol. 2012;50(2):147-51.
Ischemic stroke remains one of the top ten causes of death in children. There is evidence for the role of pro-inflammatory cytokines, such as IL-6 and the -174G>C promoter polymorphism of the IL-6 gene, in the occurrence and outcome of stroke in adults. The aim of the present study was to determine a possible association between the -174G>C IL-6 polymorphism and occurrence of paediatric stroke, its symptoms and outcome.
The study group consisted of 340 individuals: 80 stroke children, 122 parents of patients and 138 controls. The -174G/C polymorphism was genotyped using the RFLP method. For the analysis of the relationship between genotypes and stroke we used two alternative methods: the case-control model and the transmission test for linkage disequilibrium using data from families.
We observed no differences in the transmission of alleles from parents to children. We also did not find any statistical differences in distribution of genotypes and alleles between patients and controls. However, the analysis showed that post-stroke epilepsy was genotype-dependent. All children with epilepsy were G allele carriers and none of them was a CC homozygote whereas about 25% of children without epilepsy had the CC genotype.
Our study did not show any associations between the IL-6 -174 G>C polymorphism and the occurrence of stroke but we observed a relation between post-stroke epilepsy and the G allele carrier-state.
缺血性脑卒中仍然是儿童十大死因之一。有证据表明，促炎细胞因子（如 IL-6 和 IL-6 基因的-174G>C 启动子多态性）在成人中风的发生和转归中起作用。本研究旨在确定 IL-6-174G>C 多态性与儿科中风的发生、症状和结局之间是否存在关联。
研究组包括 340 人：80 名中风患儿、122 名患儿家长和 138 名对照。使用 RFLP 方法对-174G/C 多态性进行基因分型。为了分析基因型与中风之间的关系，我们使用了两种替代方法：病例对照模型和来自家庭的数据的连锁不平衡传递检验。
我们没有观察到从父母到孩子的等位基因传递的差异。我们也没有发现患者和对照组之间基因型和等位基因的分布存在任何统计学差异。然而，分析表明，中风后癫痫与基因型有关。所有癫痫患儿均为 G 等位基因携带者，无一例为 CC 纯合子，而无癫痫患儿中约有 25%为 CC 基因型。
我们的研究没有显示 IL-6-174G>C 多态性与中风的发生之间存在任何关联，但我们观察到中风后癫痫与 G 等位基因携带者状态之间存在关系。
