Gabriela Teixeira Fernanda, Mendonça Samir Andrade, Menezes Oliveira Kamilla, Barbosa Dos Santos Djanilson, Miranda Marques Lucas, Mendonça Amorim Maise, de Souza Gestinari Raquel
Laboratory of Cellular and Molecular Biology, Multidisciplinary Health Institute, Federal University of Bahia, 45029-094 Vitória da Conquista, BA, Brazil.
Health Sciences Center, Federal University of Bahia Recôncavo, 44380-000 Santo Antonio de Jesus, BA, Brazil.
Mol Biol Int. 2014;2014:490308. doi: 10.1155/2014/490308. Epub 2014 Dec 4.
Aim. Periodontitis is an inflammatory disease that affects the teeth supporting structures, triggered by periodontal pathogens, and is influenced by environmental and genetic factors. Genes encoding molecules related to the immune response, such as cytokine, are the main candidates for polymorphisms analysis and may be possibly associated with this pathology. A G/C promoter polymorphism on the IL6 gene has been shown to affect basal IL-6 levels. The aim of this study was to investigate the association between the IL6 c.-174G>C polymorphism and periodontitis in individuals from Vitória da Conquista, Bahia, Brazil. Material and Methods. Three hundred and thirty individuals (134 cases, 196 controls) were genotyped for the IL6 c.-174G>C by MS-PCR technique. Concentrations of salivary IL-6 were determined by ELISA method. Results. The IL6 c.-174G>C polymorphism was associated with periodontitis when comparing the distribution of genotypes between patients with periodontitis and control subjects. The GC genotype appeared as a protective factor for periodontitis. Results showed increased levels of salivary IL-6 in periodontitis patients. Nevertheless, there was no relationship between the concentrations of IL-6 and genotypes when comparing the case and control groups. Conclusions. Our data indicate an association between IL6 c.-174G>C polymorphism and periodontitis and showed that IL-6 may be considered an important marker for periodontitis.
目的。牙周炎是一种影响牙齿支持结构的炎症性疾病，由牙周病原体引发，并受环境和遗传因素影响。编码与免疫反应相关分子（如细胞因子）的基因是多态性分析的主要候选对象，可能与这种病理状况相关。白细胞介素6（IL6）基因启动子上的G/C多态性已被证明会影响基础IL-6水平。本研究的目的是调查巴西巴伊亚州维多利亚达孔基斯塔市个体中IL6基因c.-174G>C多态性与牙周炎之间的关联。材料与方法。采用MS-PCR技术对330名个体（134例病例，196名对照）进行IL6基因c.-174G>C基因分型。采用ELISA法测定唾液中IL-6的浓度。结果。比较牙周炎患者与对照受试者的基因型分布时，IL6基因c.-174G>C多态性与牙周炎相关。GC基因型表现为牙周炎的保护因素。结果显示牙周炎患者唾液中IL-6水平升高。然而，比较病例组和对照组时，IL-6浓度与基因型之间无关联。结论。我们的数据表明IL6基因c.-174G>C多态性与牙周炎之间存在关联，并表明IL-6可被视为牙周炎的一个重要标志物。
