Instituto de Recursos Naturales y Agrobiologia, IRNAS-CSIC, Av. Reina Mercedes 10, 41012 Sevilla, Spain.
Syst Appl Microbiol. 2012 Jul;35(5):306-9. doi: 10.1016/j.syapm.2012.04.007. Epub 2012 Jul 7.
Three actinobacteria strains isolated from a green biofilm covering the biodeteriorated interior walls of Vilar de Frades Church (Portugal) were studied using a polyphasic approach. The three strains were aerobic, non-spore forming and Gram-positive. Phylogenetically, the most closely related described species was Rubrobacter radiotolerans (94.2-94.3% and 81.9-82.5% similarities for the 16S rRNA and rpoB gene sequences, respectively). The fatty acid profile was dominated by anteiso-C(17:1) ω9c, and MK-8 was the only menaquinone present. These data clearly showed that the three strains could represent a new species, for which we propose the name Rubrobacter bracarensis sp. nov., with strain VF70612_S1(T) (=CECT 7924=DSMZ 24908) as the type strain.
从葡萄牙布拉加市维拉德弗雷德斯教堂(Vilar de Frades Church)生物降解内墙的绿色生物膜中分离到的三株放线菌菌株,采用多相分类法进行了研究。这三株菌是需氧、非孢子形成和革兰氏阳性的。系统发育分析表明,最接近的描述种是耐辐射红杆菌(Rubrobacter radiotolerans),16S rRNA 和 rpoB 基因序列的相似性分别为 94.2-94.3%和 81.9-82.5%。脂肪酸组成主要由 anteiso-C(17:1) ω9c 组成,仅存在 MK-8 一种menaquinone。这些数据清楚地表明,这三株菌可能代表一个新种,我们提议将其命名为 Rubrobacter bracarensis sp. nov.,其中菌株 VF70612_S1(T)(=CECT 7924=DSMZ 24908)为模式菌株。