Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria.
Int J Syst Evol Microbiol. 2014 Mar;64(Pt 3):705-709. doi: 10.1099/ijs.0.055152-0. Epub 2013 Oct 29.
A Gram-stain-positive, non-spore-forming bacterium (strain RV113(T)) was isolated from the marine sponge Aplysina aerophoba. 16S rRNA gene sequence analysis showed that strain RV113(T) belongs to the genus Rubrobacter, and is related most closely to Rubrobacter bracarensis VF70612_S1(T) (96.9% similarity) and more distantly related (<93%) to all other species of the genus Rubrobacter. The peptidoglycan diamino acid was lysine. Strain RV113(T) exhibited a quinone system with menaquinone MK-8 as the predominant compound. The polar lipid profile of strain RV113(T) consisted of the major compounds phosphatidylglycerol and two unidentified phosphoglycolipids. The major fatty acid was anteiso-C17 : 0ω9c. These chemotaxonomic traits are in agreement with those of other species of the genus Rubrobacter. The results of physiological and biochemical tests allowed the clear phenotypic differentiation of strain RV113(T) from all recognized Rubrobacter species. Strain RV113(T) is thus considered to represent a novel species, for which the name Rubrobacter aplysinae sp. nov. is proposed. The type strain is RV113(T) ( = DSM 27440(T) = CECT 8425(T)).
一株革兰氏阳性、非孢子形成的细菌（RV113(T) 株）从海洋海绵 Aplysina aerophoba 中分离得到。16S rRNA 基因序列分析表明，RV113(T) 株属于 Rubrobacter 属，与 Rubrobacter bracarensis VF70612_S1(T)（相似度 96.9%）最为密切相关，与属内的其他种的相似度均<93%。肽聚糖二氨基氨基酸为赖氨酸。RV113(T) 株表现出以menaquinone MK-8 为主要成分的醌系统。RV113(T) 株的极性脂谱由主要成分磷脂酰甘油和两种未鉴定的磷酸甘油酯组成。主要脂肪酸为 anteiso-C17 : 0ω9c。这些化学分类特征与 Rubrobacter 属的其他种一致。生理生化试验的结果允许 RV113(T) 株从所有公认的 Rubrobacter 种中进行明确的表型分化。因此，RV113(T) 株被认为代表一个新种，建议命名为 Rubrobacter aplysinae sp. nov.。模式菌株为 RV113(T)（ = DSM 27440(T) = CECT 8425(T)）。
