2007-2011 年葡萄牙犬诺如病毒的分子流行病学研究。
Molecular epidemiology of canine norovirus in dogs from Portugal, 2007-2011.
机构信息
Departamento de Ciências Biológicas, Laboratório de Microbiologia, Universidade do Porto, Portugal, Rua Jorge Viterbo Ferreira, 228, Porto, 4050-313, Portugal.
出版信息
BMC Vet Res. 2012 Jul 9;8:107. doi: 10.1186/1746-6148-8-107.
BACKGROUND
Canine noroviruses (NoVs) have been recently described in south European countries and associated with outbreaks of diarrhea in kennels. Unlike human NoV which are known as an important cause of acute gastroenteritis, little is known about the role of canine NoV as pathogens in dogs as well as its epidemiological features.
RESULTS
From 2007-2011, 256 stool samples were collected from dogs across Portugal and tested by RT-PCR for canine NoV. Viral fecal shedding was found to be 23% (60/256). All sequences contained the GLPSG amino acid motif characteristic of the RNA-dependent RNA-polymerase gene of NoVs and had a high nucleotide identity (range 98%-100%) to the canine NoV first described in Portugal. The highest shedding rate was detected during the winter months.
CONCLUSIONS
This study shows that canine NoV infection is endemic in the dog population of Portugal. Peak shedding was detected in the winter months, a well-known epidemiologic feature of human NoV infections.
背景
犬诺如病毒（NoV）最近在南欧国家被发现，并与犬舍爆发的腹泻有关。与已知是急性肠胃炎重要病因的人类诺如病毒不同，犬诺如病毒作为犬类病原体的作用及其流行病学特征知之甚少。
结果
2007 年至 2011 年，从葡萄牙各地的犬只中采集了 256 份粪便样本，并通过 RT-PCR 检测犬诺如病毒。发现病毒粪便脱落率为 23%（60/256）。所有序列均包含 NoV 的 RNA 依赖的 RNA 聚合酶基因的 GLPSG 氨基酸基序，与葡萄牙首次描述的犬诺如病毒具有高度核苷酸同一性（范围为 98%-100%）。最高脱落率检测到在冬季月份。
结论
本研究表明，犬诺如病毒感染在葡萄牙的犬群中流行。冬季月份检测到的脱落峰值是人类诺如病毒感染的一个众所周知的流行病学特征。
Zotero 插件