College of Life Sciences, Peking University, Beijing 100871, China [2] Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02215, USA.
Cell Res. 2012 Nov;22(11):1512-4. doi: 10.1038/cr.2012.103. Epub 2012 Jul 10.
The inside-out signaling of integrins regulates the ligand-binding affinity of the cell surface receptors in response to changes in the environment for cell survival. The specific binding to the cytoplasmic tail of integrin's β subunit by the intracellular protein talin is the key step of inside-out signaling. A "pull-push" mechanism has been proposed to explain how the PIP2-enriched membrane disrupts the dual auto-inhibition of the N-terminal talin-FERM domain by the C-terminal talin-rod domain such that activated talin-FERM can reach the β-tail for integrin activation.
整联蛋白的内信号转导通过调节细胞表面受体与配体的结合亲和力，从而响应细胞生存环境的变化。细胞内蛋白 talin 通过特异性结合整联蛋白β亚基的胞质尾，是内信号转导的关键步骤。一种“拉-推”机制被提出用来解释富含 PIP2 的膜如何破坏 N 端 talin-FERM 域对 C 端 talin-rod 域的双重自身抑制，从而使激活的 talin-FERM 能够到达β-尾以激活整联蛋白。
