Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon 402-751, Korea.
Phytochem Anal. 2013 Jan-Feb;24(1):81-6. doi: 10.1002/pca.2386. Epub 2012 Jul 9.
Glucosinolates, a class of phytochemicals found in broccoli, have attracted recent interest due to the potential health benefits associated with their dietary intake. Glucoraphanin, the most common glucosinolate in broccoli can be converted to a known cancer chemopreventive agent. Multi-phase extraction in solid-phase extraction cartridges was developed to simultaneously extract and separate this compound.
Multi-phase extraction with functionalised ionic liquid-based silica as a sorbent was used to simultaneously extract and separate glucoraphanin from broccoli.
The sorbent and broccoli sample were packed into a single cartridge, and a fixed volume of water was then used to extract and remove the target compound from the sample to the sorbent over 15 repetitions. The sorbent was then washed with n-hexane to remove any interference and the target compound was eluted with water-1% acetic acid (vol.).
Under the optimised condition, 0.038 mg/g of glucoraphanin was obtained by multi-phase extraction with 0.2 g of sorbent.
The adsorption isotherm allowed investigation of the interactions between the sorbent and target compound and provided evidence for the accuracy of this method. The low deviation error, small amount of solvents required, highly selective separation and stability of the method justify further research.
存在于西兰花中的硫代葡萄糖苷是一类植物化学物质，由于其与饮食摄入相关的潜在健康益处，近年来引起了人们的关注。西兰花中最常见的硫代葡萄糖苷——萝卜硫素，可以转化为一种已知的癌症化学预防剂。本研究采用多相萃取法，以功能化离子液体键合硅胶作为吸附剂，同时从西兰花中提取和分离萝卜硫素。
采用功能化离子液体键合硅胶作为吸附剂，通过多相萃取法同时从西兰花中提取和分离萝卜硫素。
将吸附剂和西兰花样品装入单个小柱中，然后用固定体积的水进行 15 次以上的重复提取，将目标化合物从样品中提取到吸附剂上。用正己烷洗涤小柱以去除任何干扰，然后用水-1%乙酸（体积比）洗脱目标化合物。
在优化条件下，用 0.2 g 吸附剂进行多相萃取，可得到 0.038mg/g 的萝卜硫素。
吸附等温线可以研究吸附剂与目标化合物之间的相互作用，为该方法的准确性提供了证据。该方法所需溶剂用量少、选择性高、分离效果好、稳定性高，值得进一步研究。
