Centre for Disease Control and Prevention of Guangdong province, Guangzhou.
APMIS. 2012 Aug;120(8):683-8. doi: 10.1111/j.1600-0463.2012.02890.x. Epub 2012 Mar 2.
Acute respiratory infections (ARI) are the major worldwide health problem due to associated high morbidity and mortality rates. Adenovirus (Adv) is one of the most common causes of viral ARI, and thus calls for specific diagnosis and better understanding of the epidemiology and clinical characteristics. Our aims were to find out the status of Adv infection in children <14 years with ARI, analyze the epidemiology and clinical characteristics among the Adv-infected children in Guangzhou, China, and to provide some basis for the research of Adv. The throat and pharyngeal swabs were collected among the children with acute respiratory tract infections in outpatient department from September 2006 to August 2008. The samples were analyzed by PCR and the sequences were blasted with the sequences of Adv in GenBank. Clinical data were analyzed along with virological data by using appropriate statistical methods. Adv was detected in 25 out of 512 (4.9%) children. The genome types of 23 samples were determined after analysis of the gene sequence. The most prevalent Adv type was species B type 3. Among the patients, 10 were of Ad3 (43.5%), three were of Ad1 (1.3%), five were of species C Ad2 (21.7%), and five were of species E Ad4 (21.7%). A higher incidence of positive results was found during the summer season, thus showing a pattern of seasonality. There exists Adv infection in children with acute respiratory system diseases in Guangzhou area. No significant differences were found among different age groups and gender groups. Co-infections with other respiratory virus were detected in 64% of the Adv positive samples.
急性呼吸道感染（ARI）是全球范围内的主要健康问题，因为其相关的高发病率和死亡率。腺病毒（Adv）是病毒性ARI 的最常见原因之一，因此需要进行特定的诊断，并更好地了解其流行病学和临床特征。我们的目的是了解广州地区<14 岁ARI 患儿中 Adv 感染的现状，分析 Adv 感染患儿的流行病学和临床特征，并为 Adv 的研究提供依据。在 2006 年 9 月至 2008 年 8 月期间，从门急诊患有急性呼吸道感染的儿童中采集咽拭子和咽漱液标本。采用 PCR 法对标本进行检测，并将序列与 GenBank 中 Adv 的序列进行比对。采用适当的统计方法对临床和病毒学数据进行分析。在 512 例患儿中，有 25 例（4.9%）检测到 Adv。对基因序列进行分析后，确定了 23 例样本的基因组类型。最常见的 Adv 型为 B 型 3 型。在患者中，10 例为 Ad3（43.5%），3 例为 Ad1（1.3%），5 例为 Ad2（21.7%），5 例为 Ad4（21.7%）。在夏季，阳性结果的检出率较高，呈季节性分布。在广州地区，急性呼吸系统疾病患儿中存在 Adv 感染。不同年龄组和性别组之间未见明显差异。在 64%的 Adv 阳性样本中检测到与其他呼吸道病毒的混合感染。
