肿瘤浸润中免疫成分的分类
Classification of the Immune Composition in the Tumor Infiltrate.
作者信息
Brusa Davide, Balligand Jean-Luc
机构信息
Flow Cytometry Platform, Institute of Experimental and Clinical Research (IREC), Université Catholique de Louvain (UCL), Brussels, Belgium.
Pole of Pharmacology and Therapeutics, Institute of Experimental and Clinical Research (IREC), Medical School, Université Catholique de Louvain (UCL), Brussels, Belgium.
出版信息
Methods Mol Biol. 2019;1979:305-315. doi: 10.1007/978-1-4939-9240-9_19.
Flow cytometry is one of the most suitable techniques for analyzing and classifying different cell suspensions derived from blood or others compartments. The characterization of all different cellular subtypes is made with different antibodies that detect surface or intracytoplasmic antigens. Here we describe the technique to thoroughly characterize immune cells from tumor infiltrates and proceed to isolation using single-cell sorting.
流式细胞术是分析和分类源自血液或其他区室的不同细胞悬液的最合适技术之一。使用检测表面或胞浆内抗原的不同抗体对所有不同细胞亚型进行表征。在此，我们描述了从肿瘤浸润物中全面表征免疫细胞并使用单细胞分选进行分离的技术。
Zotero 插件