Wang W-W, Ma Q, Xiang Y, Zhu S-W, Cheng B-J
School of Chemistry and Life Sciences, Suzhou University, Suzhou, Anhui, China.
Genet Mol Res. 2012 Jun 25;11(2):1690-700. doi: 10.4238/2012.June.25.2.
The receptors for the immunosuppression drugs FK506 and rapamycin are called FKBPs (FK506-binding proteins). FKBPs comprise a large family; they are found in many species, including bacteria, fungi, animals, and plants. As a class of peptidyl-prolyl cis-trans isomerase enzymes, the FKBP genes have been the focus of recent studies on plant stress tolerance and immunology. We identified and analyzed gene families encoding these proteins in maize using computational and molecular biology approaches. Thirty genes were found to encode putative FKBPs according to their FK506-binding domain. The FKBP genes can be classified into single domain and multiple domain members based on the number of the domains. By analysis of the physical locations, the 30 FKBP genes were found to be widely distributed on 10 chromosomes. After analysis of the FKBP phylogenetic tree in the maize genome, we found that the 30 genes revealed two major clades. Gene duplication played a major role in the evolution of FKBP genes, which suggests that the FKBP genes in maize have a pattern significantly different from that of these genes in rice. Based on semi-quantitative RT-PCR, we found that the 30 FKBPs were expressed differently in various tissues in maize, which suggests that FKBP genes play different roles in each tissue. Several FKBPs were expressed at higher levels in roots, indicating that these genes in maize may have similar or overlapping functions.
免疫抑制药物FK506和雷帕霉素的受体被称为FKBP（FK506结合蛋白）。FKBP构成一个大家族；它们存在于许多物种中，包括细菌、真菌、动物和植物。作为一类肽基脯氨酰顺反异构酶，FKBP基因一直是近期植物抗逆性和免疫学研究的重点。我们使用计算生物学和分子生物学方法在玉米中鉴定并分析了编码这些蛋白的基因家族。根据其FK506结合结构域，发现有30个基因编码假定的FKBP。FKBP基因可根据结构域数量分为单结构域和多结构域成员。通过对物理位置的分析，发现这30个FKBP基因广泛分布在10条染色体上。在分析玉米基因组中的FKBP系统发育树后，我们发现这30个基因显示出两个主要分支。基因复制在FKBP基因的进化中起主要作用，这表明玉米中的FKBP基因具有与水稻中这些基因显著不同的模式。基于半定量RT-PCR，我们发现这30个FKBP在玉米的不同组织中表达不同，这表明FKBP基因在每个组织中发挥不同的作用。有几个FKBP在根中表达水平较高，表明玉米中的这些基因可能具有相似或重叠的功能。
