Cherne Stephen, Popov Viorica, Feng Qinghua
Department of Pathology, University of Washington, Seattle, WA, USA.
Methods Mol Biol. 2012;903:205-23. doi: 10.1007/978-1-61779-937-2_13.
More than 100 human papillomavirus (HPV) types have been identified, and over 40 of them infect the anogenital epithelium. Because each HPV type is associated with different risks for the development of cervical cancer, detecting and genotyping HPVs has increasingly become an integral part of cervical cancer control. Here, we describe a Luminex assay-based liquid bead microarray assay for genotyping 37 HPV types, which is objective, scalable, amenable to a high-throughput configuration, and has the potential to be automated.
已鉴定出100多种人乳头瘤病毒（HPV）类型，其中40多种感染肛门生殖器上皮。由于每种HPV类型与宫颈癌发生的不同风险相关，检测HPV并进行基因分型已日益成为宫颈癌防控的一个组成部分。在此，我们描述了一种基于Luminex检测的液滴微阵列检测方法，用于对37种HPV类型进行基因分型，该方法客观、可扩展、适用于高通量配置，并且具有自动化的潜力。
