Sung Gi-Ho, Shrestha Bhushan, Han Sang-Kuk, Sung Jae-Mo
Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon 441-707, Korea.
Mycobiology. 2011 Mar;39(1):1-6. doi: 10.4489/MYCO.2011.39.1.001. Epub 2011 Mar 23.
Isolates of Ophiocordyceps heteropoda (Kobayasi) collected from Mt. Halla on Jeju-do, Korea were tested for mycelial growth on different agar media and in the presence of different carbon and nitrogen sources. Similarly, isolates were also incubated at different temperatures as well as under continuous light and dark conditions. Growth was better on Hamada agar, basal medium, and malt-yeast agar, but poor on Czapek-Dox agar. Different carbon sources such as dextrin, saccharose, starch, lactose, maltose, fructose, and dextrose resulted in better growth. Complex organic nitrogen sources such as yeast extract and peptone revealed the most effective growth. Mycelial growth was best at 25℃. The growth rate was faster in the dark than the light, but mycelial density was less compact in the dark.
从韩国济州岛汉拿山采集的异种蛇形虫草（Kobayasi）菌株，在不同的琼脂培养基上以及在不同碳源和氮源存在的情况下进行了菌丝体生长测试。同样，菌株也在不同温度以及连续光照和黑暗条件下培养。在滨田琼脂、基础培养基和麦芽酵母琼脂上生长较好，但在察氏琼脂上生长较差。不同的碳源如糊精、蔗糖、淀粉、乳糖、麦芽糖、果糖和葡萄糖能促进更好的生长。复合有机氮源如酵母提取物和蛋白胨显示出最有效的生长效果。菌丝体生长在25℃时最佳。黑暗中的生长速度比光照下快，但黑暗中的菌丝体密度较疏松。
