Hur Hyun, Imtiaj Ahmed, Lee Min Woong, Lee Tae-Soo
Department of Biology, Dongguk University, Seoul 100-715, Korea.
Mycobiology. 2008 Sep;36(3):152-6. doi: 10.4489/MYCO.2008.36.3.152. Epub 2008 Sep 30.
The fungus Phellinus is a mushroom that is widely used medicinally. The optimal conditions for mycelial growth of 13 strains of the fungus were investigated. Mycelial growth was optimal at 25℃ and was uniformly minimal at 15℃ and 35℃. Growth was optimal at pH 6~7. The mycelial phenotype was best promoted by growth using Potato Dextrose agar, Hamada, Glucose peptone, and Yeast-Malt media, whereas Czapek Dox, Hennerberg, and Lilly media were the most unfavorable for the mycelial growth of Phellinus spp. Glucose, sucrose, fructose, and dextrin were the most suitable carbon sources for mycelial growth, while lactose, maltose, and galactose were unsuitable. Among tested nitrogen sources, ammonium phosphate, potassium nitrate, and arginine best promoted mycelial growth, while alanine, urea, and histidine least promoted mycelial growth.
桑黄菌是一种广泛应用于医学领域的蘑菇。对该真菌的13个菌株的菌丝体生长的最佳条件进行了研究。菌丝体在25℃时生长最佳,在15℃和35℃时均生长最少。在pH值为6至7时生长最佳。使用马铃薯葡萄糖琼脂、滨田培养基、葡萄糖蛋白胨和酵母麦芽培养基培养时,菌丝体表型得到最佳促进,而察氏培养基、亨纳伯格培养基和利利培养基对桑黄菌属的菌丝体生长最不利。葡萄糖、蔗糖、果糖和糊精是菌丝体生长最合适的碳源,而乳糖、麦芽糖和半乳糖则不合适。在测试的氮源中,磷酸铵、硝酸钾和精氨酸对菌丝体生长的促进作用最佳,而丙氨酸、尿素和组氨酸对菌丝体生长的促进作用最小。