Shrestha Bhushan, Lee Won-Ho, Han Sang-Kuk, Sung Jae-Mo
Entomopathogenic Fungal Culture Collection (EFCC), Department of Applied Biology, Kangwon National University, Chuncheon 200-701, Korea.
Mycobiology. 2006 Jun;34(2):83-91. doi: 10.4489/MYCO.2006.34.2.083. Epub 2006 Jun 30.
Characteristic growth patterns of Cordyceps militaris isolates on various media, under varying light conditions and at varying incubation periods were examined. Light was found to be the most critical single factor in determining the density, texture, and pigmentation of the mycelial culture of the fungus. However, under the light condition, the degree of pigmentation and mycelial density were found to be affected by the incubation period and type of medium. Irrespective of the variations in medium type or incubation period, there was no pigmentation of the mycelium under dark condition. Radial growth of the mycelium was faster under dark incubation rather than under light incubation. Abundant mycelial density and darkest pigmentation of C. militaris isolates were produced in nutritionally rich media like SDAY, SMAY and CZYA, suggesting that these media may fulfill all the requirements for vegetative growth of the fungus. Growth characteristics of C. militaris isolates could be easily observed by the simple agar culture method, which would be useful to characterize the phenotypic characteristics of large number of pure cultures of the fungus under given conditions of growth factors such as medium, light and temperature.
研究了不同培养基、不同光照条件和不同培养时期下蛹虫草分离株的特征性生长模式。结果发现，光照是决定该真菌菌丝体培养物密度、质地和色素沉着的最关键单一因素。然而，在光照条件下，色素沉着程度和菌丝体密度受培养时期和培养基类型的影响。无论培养基类型或培养时期如何变化，在黑暗条件下菌丝体均无色素沉着。菌丝体在黑暗培养条件下的径向生长比光照培养条件下更快。在营养丰富的培养基如SDAY、SMAY和CZYA中，蛹虫草分离株产生了丰富的菌丝体密度和最深的色素沉着，这表明这些培养基可能满足该真菌营养生长的所有需求。通过简单的琼脂培养方法可以很容易地观察到蛹虫草分离株的生长特征，这对于在培养基、光照和温度等给定生长因子条件下表征该真菌大量纯培养物的表型特征非常有用。
