Lamsal Kabir, Kim Sang-Woo, Jung Jin Hee, Kim Yun Seok, Kim Kyoung Su, Lee Youn Su
Division of Bio-Resources Technology, Kangwon National University, Chuncheon 200-701, Korea.
Mycobiology. 2011 Mar;39(1):26-32. doi: 10.4489/MYCO.2011.39.1.026. Epub 2011 Mar 23.
Powdery mildew is one of the most devastating diseases in cucurbits. Crop yield can decline as the disease severity increases. In this study, we evaluated the effect of silver nanoparticles against powdery mildew under different cultivation conditions in vitro and in vivo . Silver nanoparticles (WA-CV-WA13B) at various concentrations were applied before and after disease outbreak in plants to determine antifungal activities. In the field tests, the application of 100 ppm silver nanoparticles showed the highest inhibition rate for both before and after the outbreak of disease on cucumbers and pumpkins. Also, the application of 100 ppm silver nanoparticles showed maximum inhibition for the growth of fungal hyphae and conidial germination in in vivo tests. Scanning electron microscope results indicated that the silver nanoparticles caused detrimental effects on both mycelial growth and conidial germination.
白粉病是葫芦科作物中最具毁灭性的病害之一。随着病害严重程度的增加，作物产量会下降。在本研究中，我们评估了银纳米颗粒在体外和体内不同培养条件下对白粉病的防治效果。在植株发病前后施加不同浓度的银纳米颗粒（WA-CV-WA13B）以测定其抗真菌活性。在田间试验中，100 ppm银纳米颗粒的施用对黄瓜和南瓜发病前后均表现出最高的抑制率。此外，在体内试验中，100 ppm银纳米颗粒的施用对真菌菌丝生长和分生孢子萌发表现出最大抑制作用。扫描电子显微镜结果表明，银纳米颗粒对菌丝生长和分生孢子萌发均产生了有害影响。
