Jo Woo-Sik, Park Ha-Na, Cho Doo-Hyun, Yoo Young-Bok, Park Seung-Chun
Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration, Daegu 702-320, Korea.
Mycobiology. 2011 Jun;39(2):129-32. doi: 10.4489/MYCO.2011.39.2.129. Epub 2011 Jun 16.
To determine the optimal media conditions for the detection of the extracellular cellulase activity in Ganoderma neo-japonicum, we varied three media conditions: dye reagent, pH, and temperature. We evaluated the use of four dyes, Congo red, phenol red, remazol brilliant blue, and trypan blue. To observe the effect of pH on the chromogenic reaction, we tested media ranging from 4.5 to 8.0. To research the effect of temperature on the clear zone and the fungus growing zone, we tested temperatures ranging from 15 to 35℃. On the whole, the best protocol called for Ganoderma neo-japonicum transfer onto media containing Congo red with a pH of 7.0, followed by incubation at 25℃ for 5 days. Our results will be useful to researchers who study extracellular enzyme activity in Ganoderma neo-japonicum.
为确定检测新日本灵芝胞外纤维素酶活性的最佳培养基条件，我们改变了三种培养基条件：染料试剂、pH值和温度。我们评估了四种染料的使用，即刚果红、酚红、瑞马唑亮蓝和台盼蓝。为观察pH值对显色反应的影响，我们测试了pH值范围为4.5至8.0的培养基。为研究温度对透明圈和真菌生长区域的影响，我们测试了15至35℃的温度范围。总体而言，最佳方案是将新日本灵芝转移到含有pH值为7.0的刚果红的培养基上，然后在25℃下培养5天。我们的结果将对研究新日本灵芝胞外酶活性的研究人员有用。
