Yoon Ji Hwan, Park Ji Eun, Suh Dong Yeon, Hong Seung Beom, Ko Seung Ju, Kim Seong Hwan
Department of Microbiology and Institute of Basic Sciences, Dankook University, Cheonan 330-714, Korea.
Mycobiology. 2007 Mar;35(1):21-4. doi: 10.4489/MYCO.2007.35.1.021. Epub 2007 Mar 31.
To evaluate which dye is effective in a plate assay for detecting extracellular cellulase activity produced by fungi, four chromogenic dyes including remazol brilliant blue, phenol red, congo red, and tryphan blue, were compared using chromagenic media. For the comparison, 19 fungal species belonging to three phyla, ascomycota, basidiomycota, and zygomycota were inoculated onto yeast nitrogen-based media containing different carbon substrates such as cellulose (carboxylmethyl and avicel types) and cellobiose labeled with each of the four dyes. Overall, the formation of clear zone on agar media resulting from the degradation of the substrates by the enzymes secreted from the test fungi was most apparent with media containing congo red. The detection frequency of cellulase activity was also most high on congo red-supplemented media. The results of this study showed that congo red is better dye than other three dyes in a plate assay for fungal enzyme detection.
为了评估哪种染料在平板试验中对检测真菌产生的细胞外纤维素酶活性有效，使用显色培养基比较了包括瑞马唑亮蓝、酚红、刚果红和胰蛋白酶蓝在内的四种显色染料。为了进行比较，将属于子囊菌门、担子菌门和接合菌门三个门的19种真菌接种到含有不同碳源（如羧甲基纤维素和微晶纤维素类型的纤维素）以及用这四种染料各自标记的纤维二糖的酵母氮基培养基上。总体而言，在含有刚果红的培养基上，由受试真菌分泌的酶对底物的降解导致琼脂培养基上形成的透明圈最为明显。在添加刚果红的培养基上，纤维素酶活性的检测频率也最高。本研究结果表明，在平板试验中检测真菌酶时，刚果红是比其他三种染料更好的染料。
