Institute of Biomembranes and Bioenergetics, National Research Council of Italy, Bari, Italy.
Front Oncol. 2012 Jul 3;2:70. doi: 10.3389/fonc.2012.00070. eCollection 2012.
Mammalian apoptosis and yeast programmed cell death (PCD) share a variety of features including reactive oxygen species production, protease activity and a major role played by mitochondria. In view of this, and of the distinctive characteristics differentiating yeast and multicellular organism PCD, the mitochondrial contribution to cell death in the genetically tractable yeast Saccharomyces cerevisiae has been intensively investigated. In this mini-review we report whether and how yeast mitochondrial function and proteins belonging to oxidative phosphorylation, protein trafficking into and out of mitochondria, and mitochondrial dynamics, play a role in PCD. Since in PCD many processes take place over time, emphasis will be placed on an experimental model based on acetic acid-induced PCD (AA-PCD) which has the unique feature of having been investigated as a function of time. As will be described there are at least two AA-PCD pathways each with a multifaceted role played by mitochondrial components, in particular by cytochrome c.
哺乳动物细胞凋亡和酵母细胞程序性死亡（PCD）具有多种共同特征，包括活性氧物质的产生、蛋白酶活性以及线粒体发挥的主要作用。鉴于此，以及酵母细胞和多细胞生物 PCD 之间的独特特征，遗传上易于操作的酿酒酵母（Saccharomyces cerevisiae）中线粒体对细胞死亡的贡献已得到深入研究。在本篇综述中，我们报告了酵母线粒体功能以及属于氧化磷酸化、线粒体内外蛋白质运输和线粒体动力学的蛋白质是否以及如何在 PCD 中发挥作用。由于在 PCD 中许多过程会随时间发生，因此我们将重点介绍基于乙酸诱导的 PCD（AA-PCD）的实验模型，该模型具有作为时间函数进行研究的独特特征。如将描述的那样，至少存在两种 AA-PCD 途径，每条途径都具有由线粒体成分（特别是细胞色素 c）发挥的多方面作用。
