Grosfeld Erika V, Bidiuk Victoria A, Mitkevich Olga V, Ghazy Eslam S M O, Kushnirov Vitaliy V, Alexandrov Alexander I
Moscow Institute of Physics and Technology, 9 Institutskiy per, Dolgoprudny, 141700 Moscow, Russia.
Federal Research Center of Biotechnology of the RAS, Bach Institute of Biochemistry, 119071 Moscow, Russia.
J Fungi (Basel). 2021 Oct 20;7(11):886. doi: 10.3390/jof7110886.
Cell death in response to distinct stimuli can manifest different morphological traits. It also depends on various cell death signaling pathways, extensively characterized in higher eukaryotes but less so in microorganisms. The study of cell death in yeast, and specifically , can potentially be productive for understanding cell death, since numerous killing stimuli have been characterized for this organism. Here, we systematized the literature on external treatments that kill yeast, and which contains at least minimal data on cell death mechanisms. Data from 707 papers from the 7000 obtained using keyword searches were used to create a reference table for filtering types of cell death according to commonly assayed parameters. This table provides a resource for orientation within the literature; however, it also highlights that the common view of similarity between non-necrotic death in yeast and apoptosis in mammals has not provided sufficient progress to create a clear classification of cell death types. Differences in experimental setups also prevent direct comparison between different stimuli. Thus, side-by-side comparisons of various cell death-inducing stimuli under comparable conditions using existing and novel markers that can differentiate between types of cell death seem like a promising direction for future studies.
细胞对不同刺激的死亡反应可表现出不同的形态特征。它还取决于各种细胞死亡信号通路，这些通路在高等真核生物中已得到广泛表征，但在微生物中了解较少。对酵母细胞死亡的研究，特别是对于理解细胞死亡而言可能是有成效的，因为已经对该生物体的多种杀伤刺激进行了表征。在这里，我们对有关杀死酵母的外部处理的文献进行了系统化整理，这些文献至少包含关于细胞死亡机制的最少数据。使用关键词搜索从7000篇论文中获取的707篇论文的数据被用于创建一个参考表，以便根据常用的检测参数对细胞死亡类型进行筛选。该表为文献检索提供了资源；然而，它也突出表明，酵母中非坏死性死亡与哺乳动物细胞凋亡之间的相似性这一普遍观点尚未取得足够进展以明确划分细胞死亡类型。实验设置的差异也妨碍了不同刺激之间的直接比较。因此，使用能够区分细胞死亡类型的现有和新型标记物，在可比条件下对各种诱导细胞死亡的刺激进行并排比较，似乎是未来研究的一个有前景的方向。
