School of Pharmacy, Fujian Medical University, Fuzhou 350122, China.
Sensors (Basel). 2018 Sep 6;18(9):2980. doi: 10.3390/s18092980.
In this work, the CdSe⁻ZnO flower-rod core-shell structure (CSZFRs) was prepared by ion-exchange method. The surface of CSZFRs was modified by 3-mercaptopropionic acid (MPA), and then the DNA probe was immobilized on the surface via chemical bond between -NH₂ of DNA probe and -COOH of MPA. Finally, the target norovirous (NV) RNA was combined with the probe according to the principle of complementary base pairing, resulting in a decrease of the photocurrent. The results show that the absorbance spectrum of visible light is enhanced for CSZFRs compared with pure ZnO. Under visible light irradiation, the photocurrent of CSZFRs is up to 0.1 mA, which can improve the sensitivity of the photoelectrochemical (PEC) biosensor. In the measurement range of 0⁻5.10 nM, the measured concentrations () have a good linear relationship with the output photocurrent of the biosensor. The linear regression equation is expressed as = 0.03256 - 0.0033 (² = 0.99, S/N = 3) with a detection limit of 0.50 nM. Therefore, this work realizes a rapid and sensitive method for the detection of NV RNA.
在这项工作中，通过离子交换法制备了 CdSe⁻ZnO 花棒核壳结构（CSZFRs）。CSZFRs 的表面通过 3-巯基丙酸（MPA）进行修饰，然后通过 DNA 探针中的 -NH₂与 MPA 中的 -COOH 之间的化学键将 DNA 探针固定在表面上。最后，根据互补碱基配对的原理，目标诺如病毒（NV）RNA 与探针结合，导致光电流减少。结果表明，与纯 ZnO 相比，CSZFRs 的可见光吸收光谱得到增强。在可见光照射下，CSZFRs 的光电流高达 0.1 mA，可以提高光电化学（PEC）生物传感器的灵敏度。在 0⁻5.10 nM 的测量范围内，测量浓度（）与生物传感器的输出光电流之间具有良好的线性关系。线性回归方程表示为 = 0.03256 - 0.0033（²= 0.99，S/N = 3），检测限为 0.50 nM。因此，这项工作实现了一种快速灵敏的 NV RNA 检测方法。
